突变型p53与胃癌多重耐药基因表达的相关性研究  

Study on relationship between mutant p53 and multidrug resistance gene 1 in gastric cancer

作　　者：谭亚君[1] 谢鑫友[2] 陈瑜[1] 

机构地区：[1]浙江大学医学院附属第一医院检验科,浙江杭州310003 [2]浙江大学医学院附属邵逸夫医院,浙江杭州310016

出　　处：《实用肿瘤杂志》2006年第4期309-311,共3页Journal of Practical Oncology

摘　　要：目的研究突变型p 53与M DR-1基因间相互关系,探讨p 53途径对防治肿瘤细胞多重耐药的意义。方法取胃癌组织切片,采用免疫组化方法检测组织切片p53蛋白和M DR-1基因蛋白产物Pg糖蛋白,分析突变型p 53与M DR-1基因表达产物的相互关系。将突变型p 53、sv40T ag(封闭p 53)导入胃癌细胞株中,观察胃癌细胞株中M DR-1基因mRNA表达的变化。结果46例胃癌组织中p53蛋白阳性率为60.9%,Pg蛋白阳性率为73.9%。在28例p53蛋白阳性表达中有23例(82.1%)Pg蛋白表达阳性。18例p53蛋白阴性表达中9例(50.0%)为Pg蛋白表达阳性。p53和Pg蛋白之间的相关性有显著性意义(P<0.05)。导入突变型p 53细胞组的M DR-1基因mRNA表达比导入突变型p 53+sv40T ag细胞组、对照组M DR-1基因mRNA表达增强。结论突变型p 53可能促进肿瘤细胞M DR-1基因表达,使肿瘤细胞更易产生耐药。Objective To investigate the relationship between mutant p53 and multidrug resistant gene 1 （MDR-1） in gastric cancer. Methods TissQe sections were obtained from 46 cases of gastric cancer. The level of p53 protein and MDR-1 gene product （PgP） were examined using immunohistochemical techniques. Recombinant mutant p53 （mp53） and sv40Tag genes were constructed. The recombinant rap53 and mp53＋sv40Tag were transferred to gastric cancer cells, the expression of MDR-1 mRNA was detected with RT-PCR. Results Positive p53 was detected in 60. 9%（28/46） samples and positive PgP in 73.9% （34/46） samples; and Pgp expression was detected in 23 of 28 p53-positive samples, and 9 of 18 p53-negative samples; p53 expression was positively associated with PgP （P〈0. 05）. Cancer cells transfected with mutant p53 showed higher MDR-1 mRNA than cancer cells transfected with mp53＋sv40Tag and control. Conclusion The results demonstrate that mutant p53 may accelerate the MDR-1 gene expression.

关 键 词：胃肿瘤/病理学 基因 p53 P-糖蛋白类/生物合成 药物耐受性/遗传学 肿瘤细胞 培养的 

分 类 号：R735.2[医药卫生—肿瘤]