人骨髓间充质干细胞体外内皮细胞诱导分化的研究(英文)  

Human bone marrow mesenchymal stem cells differentiate toward endothelial lineagein vitro

作　　者：郭新[1] 李玉林[1] 何旭[1] 李伟[1] 李一雷[1] 张丽红[1] 杨瑞光[1] 赵维[1] 

机构地区：[1]吉林大学基础医学院病理生物学教育部重点实验室,吉林长春130021

出　　处：《中国病理生理杂志》2006年第8期1586-1590,共5页Chinese Journal of Pathophysiology

基　　金：theDoctor’sspecificgrant,ministryofeducation(No.20021083064)

摘　　要：目的:探讨人骨髓间充质干细胞hMSCs(hum an m esenchym al stem cells,hMSCs)体外内皮分化基础及其诱导条件。方法:采用密度分离与贴壁筛选结合法分离hMSCs,体外联合应用生长因子VEGF165和不同细胞外基质纤维连接蛋白(FN)与Ⅰ型胶原(Col),对其进行内皮诱导分化。通过免疫细胞化学、细胞化学、流式细胞分析法及透射电镜对其分化后细胞进行鉴定。结果:hMSCs表达早期内皮分化标志之一KDR;PAS反应阳性及超微结构显示,hMSCs胞浆内含有大量糖原形成糖原池,分化后细胞胞浆外质内糖原明显减少或消失,暗示细胞发生分化;细胞诱导后CE34、β1整合素和KDR表达均增强。结论:诱导后细胞为过渡细胞群类型(transit popu lation,TP),可向内皮前体细胞(endothelial progen itor cells,EPC)EPC方向分化。推测由此类型细胞再分化为内皮细胞(endothelial cells,ECs),即hMSCs→TP→EPC→ECs。AIM ： To investigate the cytological basis and differentiating conditions of human bone marrow mesenchymal stem cells（hMSCs） differentiated into cells of the endothelial lineage in vitro. METHODS： hMSCs were isolated by density gradient centrifugation and fractionated on a 1 073 g/L Percoll. The combination of VEGF165 and various matrix proteins including fibronectin （FN） and type Ⅰ collagen （Col） was used to induce hMSCs in vitro. Cells were characterized by immunohistochemistry, cytochemistry, FACS and ultrastructure to identify and detect the differentiated populatiovi and markers, RESULTS： hMSCs was positive for KDR. PAS reaction was positive and ultrastructure of hMSCs showed glycogen, pool in ectoplasm. Glycogen reducing or disappear suggested that stem cells have occurred differentiation. Induction of hMSCs resulted in the increase of KDR, β1 integrin and CD34. CONCLUSION： hMSCs were induced to a transit population （ TP） that differentiated toward the endothelial progenitor cells （ EPC ）, but not a really EPC. hMSCs pedigree diagram of differentiation was hMSCs→TP→EPC→endothelial cells （ECs）.

关 键 词：骨髓间质干细胞 内皮细胞 内皮生长因子 纤维蛋白类 胶原Ⅰ型 

分 类 号：R363[医药卫生—病理学]