BABL/C×C57BL/6杂交F1代小鼠H22肝癌实体瘤模型的建立  

Establishment of Mouse Solid Tumor Experimental Model of Hepatoma 22 in Hybrid F1 Mice of BABL/C×C57BL/6

作　　者：李晓峰[1] 叶韵斌[1] 陈强[1] 范南峰[1] 黄炜伟[1] 陈明水[1] 

机构地区：[1]福建医科大学教学医院福建省肿瘤医院内科,福州350014

出　　处：《福建医科大学学报》2006年第4期387-390,共4页Journal of Fujian Medical University

基　　金：福建省自然科学基金资助项目(C0310035);福建省医学创新课题(2003-CX-16)

摘　　要：目的 探讨H22细胞在BABL／CXC57BL／6杂交F1代小鼠的成瘤性，为研究移植物抗肿瘤效应和移植物抗宿主病提供实验对象。方法 BABL／C来源的H22肝癌细胞接种于杂交F1小鼠皮下，观察成瘤状况，并用流式细胞仪检测H22细胞的主要组织相容抗原复合物分子。结果 每只F1小鼠接种2×10^6个H22细胞时，成瘤率为100％，无自发缓解；接种第14天，肿瘤体积的变异系数23％～34％；H22细胞的H-2K。表达率（75．70±9．32）％。结论 H22细胞可成功地接种于F1小鼠皮下成瘤，建立杂交F1代实体瘤动物模型。Objective To establish a mice solid＇ tumor model of hepatoma 22 in hybrid F1 mice of BABL/C;K C57BL/6 for in vivo study of graft-versus-tumor（GVT） and graft-versus-host disease（GVHD）induced by major histocompatibility complex（MHC） half-matched bone marrow or stem cell transplanta- tion. Methods Subcutaneous injection of H22 cells, which were cultured in the abdominal cavity of BABL/C mouse （H-2Kd） into F1 mice （H-2Kd/b）, derived from BABL/C in hepatoma cell, and the growth of solid tumor was observed. The MHC phenotypes of H22 cells were assayed by the flow cell analysis system（FCAS）. Results Subcutaneous injection of 2 × 10^6 H22 cells per mouse caused 100 percent of hapatoma genesis in F1 mice. The expression rate of H-2Ka on H22 cells was （75. 70 ± 9. 32）%. Conclusion A solid tumor experimental model was established successfully in hybrid F1 mice.

关 键 词：H22肝癌细胞 肝肿瘤 实验性 疾病模型 动物 小鼠 

分 类 号：R735.7[医药卫生—肿瘤]