莲房原花青素改善小鼠学习记忆障碍的研究  

EFFECTS OF PROCYANIDINS EXTRACTED FROM LOTUS SEEDPOD ON LEARNING AND MEMORY IMPAIRMENT IN MICE

作　　者：龚玉石[1] 唐瑛[2] 肖俊松[1] 谢笔钧[1] 

机构地区：[1]华中农业大学食品科技学院,武汉430070 [2]广州军区武汉总医院医学实验中心,武汉430070

出　　处：《营养学报》2006年第4期318-321,共4页Acta Nutrimenta Sinica

基　　金：博士点基金(No.20040504013);国家自然科学基金(No.30270938)

摘　　要：目的:研究莲房原花青素(LSPC)抑制东莨菪碱和乙醇致小鼠学习记忆障碍的作用,并探讨其作用机制。方法:100只昆明小鼠分两批(50,50)进行实验,每批实验皆为5组,即正常对照组、模型组、22.5、45、90mg/kgbwLSPC组,每组10只,分别灌胃等量生理盐水、生理盐水、LSPC22.5、45、90mg/kgbw。20d后制备学习记忆障碍模型。按0.1ml/10gbw分别i.p.3mg/kgbw东莨菪碱或40%乙醇,采用Y迷宫法测定LSPC对小鼠学习记忆的影响。对于乙醇诱导的学习记忆障碍小鼠,Y迷宫结束后,取脑组织,采用试剂盒检测脑组织中丙二醛(MDA)、超氧化物歧化酶(SOD)和B型单胺氧化酶(MAO-B)的含量及活性。结果:22.5和90mg/kgbwLSPC对东莨菪碱致学习记忆障碍小鼠有显著的改善作用。而三种不同剂量的LSPC对乙醇致学习记忆障碍小鼠均有显著的改善作用;而且可减少小鼠脑中MDA的含量、提高SOD活性和降低MAO-B活性。结论:LSPC对东莨菪碱和乙醇诱导的小鼠学习记忆障碍都有很好的改善效果。对于乙醇诱导的小鼠学习记忆障碍,LSPC的改善作用机制可能与抑制乙醇引起的小鼠脑中MAO-B活性的升高,同时减少神经元的氧化损伤有关。Objective： To study the effects of LSPC on impairment of learning and memory in mice induced by scopolamine and alcohol, and its possible mechanism. Method： One hundred male Kun-Ming mice were randomly divided into 2 batches, 50 in each. Each batch was then divided into 5 groups （n=10）： control, model, LSPC of 22.5, 45, 90 mg/ kg bw. Group 3-5 was treated with lotus seedpod procyanidins （LSPC, dissolved in normal saline, 22.5, 45, 90 mg/kg bw） by ig. each day for 20 d, while group 1-2 was treated with ig. normal saline. The model of learning and memory impairment was established by ip.3 mg/kg bw scopolamine hydrobromide or ig. 40% alcohol in 0.1 ml/10 g bw respectively. Then, effect of LSPC on learning and memory in mice were tested by Y-maze. As to the mice whose learning and memory ability was impaired by alcohol, their brains were dissected out after Y-maze experiment for detecting the level of MDA, the activity of SOD and MAO-B. Results： The learning and memory abilities of mice impaired by scopolamine were remarkably improved after 22.5 and 90mg/kg bw LSPC treatment. In the alcohol experiment, the impairment was significantly improved after three different doses of LSPC administration. The level of MDA was reduced, the activity of SOD increased and the activity of MAO-B decreased in mice that learning and memory was impaired by alcohol. Conclusion： LSPC can improve learning and memory abilities of the mice impaired by alcohol and scopolamine, and the mechanism in alcohol experiment probably lay on its suppressive effect on MAO-B activity and protection of neurons from oxidative stress.

关 键 词：莲房原花青素(LSPC) 学习记忆 超氧化物歧化酶 丙二醛 B型单胺氧化酶 

分 类 号：R151[医药卫生—营养与食品卫生学]