孕期膳食补锌对胎盘及母鼠小肠锌转运体mRNA表达谱的影响  

EFFECT OF ZINC SUPPLEMENTATION ON THE EXPRESSION PROFILE OF ZINC TRANSPORTERS mRNA IN PLACENTA AND SMALL INTESTINE OF PREGNANT RATS

作　　者：谢良民[1] 黄昕[1] 葛懿云[1] 厉曙光 汤家铭[3] 李俊旋[1] 

机构地区：[1]同济大学附属东方医院营养中心,上海200120 [2]同济大学基础医学院预防医学教研室,上海200092 [3]上海中医药大学实验动物中心,上海201203

出　　处：《营养学报》2006年第4期335-337,341,共4页Acta Nutrimenta Sinica

基　　金：上海市科学技术发展基金(No.03JC14072)

摘　　要：目的:探讨孕期膳食补锌对胎盘及母鼠小肠中ZnT1、2、5和6mRNA表达谱的影响。方法:18只SD孕鼠随机分为正常对照组(ZC)和补锌组(ZS)。两组进食常规饲料,ZC组(n=9)饮用去离子水,ZS组(n=9)饮用补锌水(含锌1.26mmol/L)。分别于孕D9.5和孕D17.5时取胎盘和小肠。以半定量逆转录聚合酶链反应(RT-PCR)测定胎盘和母鼠小肠中ZnT1、2、5和6mRNA的相对表达量。结果:在孕D9.5时,孕期膳食补锌可显著上调胎盘ZnT1、2及小肠ZnT1、2和6mRNA的表达,下调胎盘ZnT5mRNA的表达,对胎盘ZnT6及小肠ZnT5mRNA的表达则无影响;在孕17.5d时,膳食补锌可显著上向调控胎盘ZnT5和ZnT6mRNA的表达,对胎盘及小肠ZnT1、2mRNA的表达无影响,小肠ZnT5mRNA检测不到。结论:妊娠不同时期膳食不同锌摄入水平显著影响胎盘和母体小肠中锌转运体mRNA的表达谱。Objective： To investigate the effect of zinc supplementation on the expression profile of zinc transporters mRNA in placenta and small intestine of pregnant rats. Method： Eighteen pregnant SD rats were randomly divided into normal control （ZC） and zinc supplement group （ZS）. Rats in ZC group drank deionized distilled water while those in ZS group drank water supplemented with zinc of 1.26 mmol/L. Placenta and small intestine were taken at gestational day 9.5 and 17.5, respectively. The expression of ZnT1, 2, 5 and 6 mRNA was examined by RT-PCR. Results： At gestational D9.5, the expression of placental ZnT1, 2 and small intestinal ZnT1, 2, 6 mRNA was up-regulated, and placental ZnT5 mRNA down-regulated by dietary zinc supplementation. Dietary zinc intake had no effect on the expression of placental ZnT6 and small intestinal ZT5 mRNA. At gestational D17.5, the expression of placental ZnT5 and 6 mRNA was up-regulated by dietary zinc supplementation, and dietary zinc had no effect on the expression of placental and small intestinal ZnT1, 2 mRNA. The expression of ZnT5 mRNA at gestational D17.5 in both groups was not detectable. Conclusion： Dietary zinc supplementation during pregnancy has significant effect on the expression profile of ZnT 1, 2, 5 and 6 mRNA in placenta and small intestine of pregnant rats.

关 键 词：锌 胎盘 小肠 ZnT1 ZnT2 ZnT5 ZnT6 

分 类 号：R151[医药卫生—营养与食品卫生学]