N^+离子注入阿维拉霉素产生菌诱变效应的研究  

Study on mutagenic effects of avilamycin-producing strain induced by N^+ ion implantation

作　　者：朱传合[1] 贺亚男[1] 路福平[1] 杜连祥[1] 

机构地区：[1]天津科技大学生物工程学院天津市工业微生物重点实验室,天津300222

出　　处：《核技术》2006年第8期609-613,共5页Nuclear Techniques

摘　　要：选用低能(30keV)N+离子注入阿维拉霉素产生菌SV-56,研究其诱变效应。试验结果表明:SV-56菌株存活率曲线是典型的“马鞍型”剂量-效应曲线,“马鞍型”区域内具有高的正突变率(20．9％-26．2％)。通过5×1015cm-2N+注入诱变处理、链霉素抗性筛选,最终获得一株稳定性良好、阿维拉霉素产量稳定在79．6-82．9 mg·L-1之间、较出发菌株提高41．4％-47．2％的突变株SVN-116。Mutagenic effects of avilamycin-producing strain induced by N^＋ ion implantation have been investigated. The experimental results indicated that the survival rate curve took a ＂saddle＂ shape and the unique mutation effects of N^＋ ion implantation were presented. To obtain high-yield avilamycin-producing strain, the ion implantation dose of 5×10^15 cm^-2（the mutation rate was 20.9%-26.2%） was used and screening of streptomycin-resistant mutant was done in the study on breeding mutation. The SVN-116 mutant was obtained, with an avilamycin yield of 79.6-82.9 mg·L^-1 that was 1.414-1.472 times as much as the original strain.

关 键 词：离子注入 阿维拉霉素 诱变选育 诱变效应 

分 类 号：Q933[生物学—微生物学] R978.1[医药卫生—药品]