用气相色谱法研究淡紫拟青霉对辛硫磷的降解作用  

Determination of Phoxim Degradation by Paecilomyces lalicinus Using Gas Chromatography

作　　者：黄素芳[1] 李芳[2] 朱育菁[1] 刘波[1] 

机构地区：[1]福建省农业科学院生物技术研究所,福建福州350003 [2]福建农林大学植物保护学院,福建福州350002

出　　处：《农业环境科学学报》2006年第4期1010-1013,共4页Journal of Agro-Environment Science

基　　金：国家863计划项目(2002AA244031-2);福建省计委项目(闽计投资[2003]170);福州市贸易发展局无公害蔬菜研究项目

摘　　要：采用气相色谱法研究了淡紫拟青霉对有机磷农药辛硫磷的降解作用,并建立了从真菌菌液中检测辛硫磷残留的分析方法。结果表明,添加浓度为400μg·mL-1的辛硫磷经过淡紫拟青霉菌5d降解,降解率达100%。采用石油醚/丙酮(1∶1)为提取液,置40℃,110r·min-1振荡30min,石油醚萃取,浓缩,石油醚定容,经气相色谱测定辛硫磷残留量方法,其方法添加回收率范围为93%～99%,变异系数<5%。Gas chromatography was used to determine the degradation of phoxim by Paecilomyces lalicinus, NH-PL-03 strain, and a method was established for detecting residues of phoxim from fermentation＇s liquid. The result showed that mixed Paecilomyces lalicinus cultured with phoxim 400 μg·mL^-1 for 5 d, the phoxim were quickly degraded, with the degradation rate being 100%, and the degradation rates decreased with the concentration increase of added phoxim. Phoxim was extracted with a mixture of petrolic/acetone （ 1：1 V）as extraction sol- vent, treated at 40 ℃, 110 r·min^-1 for 30 min, blowtryed and concentrated by N2, and determined by GC. The results of analysis indicated that the average recovery rates ranged from 93% to 99% with the variation coefficient less than 5% （n=6） and the detection limit of phoxim residue was 3 ng·mL^-1.

关 键 词：淡紫拟青霉 辛硫磷 气相色谱 残留检测方法 

分 类 号：X839.2[环境科学与工程—环境工程]