C3d对原癌基因HER-2/neu基因免疫的调节作用  

The regulation on the immune responses against proto-oncogene HER-2/neu following DNA vaccination by C3d molecule

作　　者：关庆东[1] 倪晶[1] 王立新[1] 乔滨[1] 储以微[1] 熊思东[1] 

机构地区：[1]复旦大学上海医学院免疫学系教育部分子医学重点实验室上海基因免疫与疫苗研究中心,上海200032

出　　处：《中国免疫学杂志》2006年第8期727-731,共5页Chinese Journal of Immunology

基　　金：上海市科技攻关重点项目(03dz19229);国家重点基础研究发展计划(2001CB510006);国家杰出青年科学基金研究计划(39925031)资助

摘　　要：目的:研究C3d对HER2/neu基因疫苗诱导的免疫应答的调节作用。方法:分别构建含人HER2/neu胞外区(hECD)的重组质粒TR421hECD和hECD与C3d的融合质粒TR421hECDC3d3、pcDNA3hECDC3d3。以质粒基因免疫小鼠,定期收集血清,以ELISA法检测抗HER2/neu抗体,3HTdR掺入法检测细胞免疫应答,观察C3d对hECD免疫应答的调节作用,并以C3d对HBV免疫应答的调节作为对照。结果:hECDC3d3融合质粒诱导的抗HER2/neu抗体水平显著低于hECD质粒诱导的抗体水平,其诱导的淋巴细胞增殖反应也显著低于hECD质粒免疫组,但C3d能增强HBV基因免疫诱导的免疫应答。结论:C3d负调控HER2/neu基因免疫诱导的免疫应答。Objective：To investigate whether C3d can regulate the specific immune responses against HER-2/neu induced by directly injection of naked plasmids DNA containing three tandem repeats of C3d and the extracellular domain of HER-2/neu（hECD）. Methods：Recombinant plasmids TR421-hECD encoding hECD gene, TR421-hECD-C3d3 and peDNA3-hECD-C3d3 encoding hECD-C3d3 fusion gene were constructed. Female BALB/c mice were immunized with these recombinant plasmids three times at three weeks interval. The levels of specific IgG in sera collected at the indicated times from each group were determined by ELISA and specific cel-lular responses were analyzed by 3H-TdR proliferation assay. As a control, the regulation of C3d on the immune responses against by HBV was evaluated. Results：C3d could enhance the immune responses challenged by HBV. However, the specific antibody response against hECD in the groups primed with hECD-C3d3-DNA was significant lower than that in hECD-DNA groups（ P 〈 0. 05 ）. And the specific lympho-proliferative response in the mice immunized with hECD-C3d3-DNA was also significant lower than that in hECD-DNA group（ P 〈0. 05）. Conclusion ：These results indicate that three tandem repeats of C3d could negatively regulate the immune responses against HER-2/neu induced by gene immunization.

关 键 词：C3D HER-2/NEU 基因免疫 负调控 

分 类 号：R392.12[医药卫生—免疫学]