神经节苷脂对表皮郎格罕氏细胞抗原提呈功能调节的体内外研究  

Ganglioside inhibit the antigen-presenting capability of epidermal Langerhans cells

作　　者：孙晶[1] 李玉林[2] 姜日花[1] 李一雷[2] 

机构地区：[1]吉林大学中日联谊医院皮肤科,长春130031 [2]吉林大学病理生物学教育部重点实验室,长春130021

出　　处：《中国免疫学杂志》2006年第8期735-737,742,共4页Chinese Journal of Immunology

摘　　要：目的:探讨神经节苷脂对表皮郎格罕氏细胞抗原提呈作用的影响。方法:体外将神经节苷脂(GD1a)与表皮郎格罕氏细胞(Lc)、钥孔戚血蓝素(KLH)及T细胞克隆系HDK1混合培养后,通过ELISA的方法来检测培养液上清中的IFNγ水平。体内利用肿瘤相关抗原(TAA)皮下注射到小鼠背部皮下,每周1次,连续3周,使小鼠致敏,第4周对小鼠进行激发试验,刺激产生迟发型变态反应(DTH),即将表皮郎格罕氏细胞(Lc)与神经节苷脂(GD1a)和肿瘤相关抗原(TAA)共同孵育后注射到小鼠后足掌侧,分别于注射后24和48小时测量足掌肿胀程度来评估神经节苷脂(GD1a)对小鼠迟发型变态反应(DTH)的作用。体内、体外试验均设定阴性对照组。结果:体外实验中神经节苷脂(GD1a)可明显抑制HDK1分泌产生IFNγ,体内试验表明神经节苷脂(GD1a)可降低小鼠足掌肿胀程度,抑制小鼠迟发型变态反应。结论:神经节苷脂(GD1a)具有抑制表皮郎格罕氏细胞抗原提呈作用。Objective：To study whether gangliosides inhibit the antigen-presenting capability of epidermal Langerhans cells. Methods：In the vitro test, the purified Langerhans cells were exposed to increasing concentration of gangliosides for 5 hours at 37℃, then KLH was added and incubated for2 hours at 37℃. At last we added HDK1 and after 72 hours of coculture, levels of IFN-γ in culture supematants were measured by ELISA. In the vivo test, immunity to the S1509a spindle cell carcinoma was induced by s.c. inoculation at weekly intervals into naive syngeneic（ CAF1 ） for a total of three immunizations. Delayed-type hypersensitivity（DTH） was elicited 1 week after the last immunization by injection a hind footpad with TAA-pulsed Langerhans cells incubated with or without gangliosides. Footpad swelling was assessed at 24 and 48 hours with a spring-loaded engineer＇s micrometer. Results ：The presence of gangliosides during HDK1 activation reduced the expression of IFN-γ in vitro test. Gangliosides suppressed Langerhans cells to elicit DTH against TAA in vivo test. Conelusion：Ganglioside inhibit the antigen-presenting capability of epidermal Langerhans cells.

关 键 词：神经节苷脂 郎格罕氏细胞 抗原提呈 

分 类 号：R741[医药卫生—神经病学与精神病学]