组织工程化神经修复周围神经缺损的实验研究  

Experiment of tissue engineering bioartificial nerve graft in repair of peripheral nerve

作　　者：利春叶[1] 廖全全[1] 张洪[2] 彭爱军[1] 李文萍[3] 

机构地区：[1]南方医科大学附属深圳宝安医院骨II科,广东深圳518101 [2]深圳市第二人民医院骨科,广东深圳518035 [3]广州医学院附属广东省妇儿医院,广东广州510010

出　　处：《中国现代医学杂志》2006年第15期2308-2310,共3页China Journal of Modern Medicine

摘　　要：目的通过人工神经修复神经缺损的实验研究,为神经组织工程的进一步研究和临床应用提供实验依据。方法分3组修复大鼠1cm长坐骨神经缺损:(A组)含许旺细胞的移植体;(B组)无许旺细胞的移植体;(C对照组)自体神经移植。术后10周内,进行肌肉湿重、电生理等测定以及组织学观察、图像分析。结果许旺细胞能与PLGA纤维构成活性细胞-材料复合物;神经修复术后10周,A、C两组电生理特性、组织学变化以及肌肉湿重等测定均明显优于B组,A组与C组之间无显著差别。结论许旺细胞能与PLGA纤维在体外构建具有活性的神经移植体并能够促进神经再生,其修复效果接近于自体神经移植。[Objective] Through the experiment of using bioartificial nerve substitute for nerve autograft to repair the nerve defect, we expect to provide for further study of tissue engineering ioartificial nerve and its clinical practice. [Methods] Rat＇s sciatic nerve defect being repaired according to the following three ways： conduits with Schwann cells（group A）; conduits without Schwann cells （group B） and autologous nerves （group C）. Evaluation of its feasibility for promoting nerve regeneration included general observation, muscle measurement, electrophysiological examination, histomorphologically analysis was done after 10 weeks of operation. [Results] Schwann cells and the PLGA filament could form cell-biomaterials complex body. At the end of 10 weeks after operation, the relative weight of the triceps surae muscle, myelinated fibers, the AMP, AREA, NCV of regenerated nerve of group A and C were significantly better than group B, and there was no significant difference compared to group C. [Conclusion] It was showed that PLGA was compatible with Schwann cells and suitable to construct tissue-engineered bioartificial nerve.and its effect of repairing the nerve defect was similar to autologous nerves.

关 键 词：组织工程 许旺细胞 神经移植体 周围神经再生 大鼠 

分 类 号：R-332[医药卫生]