脂肪乳剂建立高脂血症模型小鼠的比较研究  

Comparative Study On Hyperlipemia Model by Fat Emulsion in Mice

作　　者：熊静悦[1] 周桢昊[1] 赵璐[1] 杨旋[1] 曾南[1] 

机构地区：[1]成都中医药大学药学院,四川成都611731

出　　处：《中医药学刊》2006年第8期1507-1508,共2页Study Journal of Traditional Chinese Medicine

摘　　要：目的:探讨、完善脂肪乳剂制备小鼠高脂血症模型的方法。方法:采用胆固醇与猪油不同比例配方的脂肪乳剂连续灌胃给予小鼠7天,及停脂肪乳剂7天后,检测小鼠血清中胆固醇和甘油三酯的含量。结果:胆固醇20%、猪油40%比例的脂肪乳剂配方连续灌胃7天,可明显提高小鼠血清胆固醇和甘油三酯含量,并以升高甘油三酯水平作用显著,但停脂肪乳剂后7天血脂水平即恢复正常,不能维持;胆固醇10%和15%、猪油20%和30%比例的脂肪乳剂配方升高血脂水平作用不明显。结论:脂肪乳剂中胆固醇和猪油的含量高低是制备高脂血症的关键,此外实验动物种类、实验的操作及其他试剂的质量亦为影响因素。Objective：To investigate the procedure of the hyperlipemia model by intragastric administration of fat emulsion in mice. Methods： Three kinds of fat emulsion that were mainly composed of cholesterol and lard in different proportion were intragasrricly administrated for 7 days to make hyperlipemia model in mice. The serum concentration of cholesterol and triglyceride in mice were detected on 8th day and 15th day. Results： The fat emulsion that was mainly composed of 20% cholesterol and 40% lard, significantly increased the serum contents of cholesterol and triglyceride in mice, especially for triglyceride, however it could not keep. Other two kinds of fat emulsion that were composed of 10% /20% cholesterol and 15%/30% lard separately had no increasing effects. Conclusion： The proportion of cholesterol and lard in fat emulsion play a key role on preparing hyperlipemia model in mice, meanwhile the species of animals, the experimental operation and the quality of reagent were also important.

关 键 词：脂肪乳剂 高脂血症 动物模型 

分 类 号：R-332[医药卫生]