小麦生长点转化法初报  

PRELIMINARY STUDY ON TRANSFORMATION OF WHEAT APICAL POINT

作　　者：杜立群[1] 李银心[1] 麻密[1] 张志宏[1] 朱至清[1] 龚蓁蓁[2] 

机构地区：[1]中国科学院植物研究所,北京100093 [2]中国科学院生物化学研究所,上海200031

出　　处：《Acta Botanica Sinica》1996年第11期921-924,共4页Acta Botanica Sinica（植物学报：英文版）

基　　金：中国科学院重大基金

摘　　要：Apical points of young seedlings of wheat (Triticum aestivum) cultivar “Jing 411” and somatic calli of cultivar “FK8” were transformed with plasmids pBI121 and (or) pBIAH A + by using microprojectile bombardment. Histochemical assay of GUS activity showed positive reaction on some of the transformation processed apical points and calli. This demonstrated that foreign genes were introduced into the apical meristematic cells as well as the callus cells. The plantlets of cv. “Jing 411” survived after apical point transformation with pBIAH A + were transplanted into the field and the progenies were screened with kanamycin. 4% of the screened seeds germinated into green seedlings with kanamycin resistance. Dot hybridization of total DNA from kanamycin resistant plants showed the existence of foreign DNA in some of the detected plants.Apical points of young seedlings of wheat (Triticum aestivum) cultivar “Jing 411” and somatic calli of cultivar “FK8” were transformed with plasmids pBI121 and (or) pBIAH A + by using microprojectile bombardment. Histochemical assay of GUS activity showed positive reaction on some of the transformation processed apical points and calli. This demonstrated that foreign genes were introduced into the apical meristematic cells as well as the callus cells. The plantlets of cv. “Jing 411” survived after apical point transformation with pBIAH A + were transplanted into the field and the progenies were screened with kanamycin. 4% of the screened seeds germinated into green seedlings with kanamycin resistance. Dot hybridization of total DNA from kanamycin resistant plants showed the existence of foreign DNA in some of the detected plants.

关 键 词：小麦 生长点 基因枪 遗传转化 

分 类 号：S512.103.2[农业科学—作物学]