Initial Study on Immune Escape Mechanism of Mouse Acute Myelomonocytic Leukemic Cell Line WEHI-3  

作　　者：黎纬明[1] 刘凌波[1] 何伟 邹萍[1] 

机构地区：[1]武汉华中科技大学同济医学院附属协和医院血液病研究所,430022

出　　处：《The Chinese-German Journal of Clinical Oncology》2006年第4期291-293,共3页中德临床肿瘤学杂志（英文版）

基　　金：Supported by a grant from National Natural Sciences Foundation of China (30240022).

摘　　要：Objective： To investigate the expression of Fas, Fas ligand （FasL） and CD80 on the cell surface of mouse acute myelomonocytic leukemia cell line WEHI-3 and the function of FasL. Methods： The expression of Fas, FasL and CD80 was detected on WEHI-3 cell surface by flow cytometry. Simultaneously the function of FasL was determined by Thymidine （^3H-TdR） Incorporation. Results： The expression of CD80 and Fas on WEHI-3 cell surface was 5.06%±0.41% and 6.75%±2.31% （n=5） respectively, and the expression of FasL was up to 63.73%±5.23% （n=5）. The apoptotic rate of YAC-1 cells was 26%±4.5%, 35%±3.2% and 43%±2.7% （n=5） respectively when WEHI-3 （effector cell, E） and Fas^＋ YAC-1 cells （target cell, T） were cultured in the ratio of 3：1, 10：1 and 30：1. Conclusion： WEHI-3 cells express high FasL, low Fas and CD80, and can induce apoptosis of Fas^＋ YAC-1 cells.

关 键 词：acute myelomonocytic leukemia FASL FAS CD80 immune escape 

分 类 号：R733.7[医药卫生—肿瘤]