肝动脉阻断对大鼠种植性肝癌的HIF-1α及VEGF表达的影响  

Effect of Hepatic Arterial Blockage on HIF-1α and VEGF Expression of Transplanted Hepatoma in Rats

作　　者：王勇[1] 冯敢生[1] 钱骏[1] 尹军[1] 夏风[1] 

机构地区：[1]华中科技大学附属协和医院介入放射科,武汉430022

出　　处：《临床放射学杂志》2006年第8期775-777,共3页Journal of Clinical Radiology

摘　　要：目的探讨肝肿瘤行肝动脉栓塞术后肿瘤组织缺氧产生相关的分子生物学改变。材料与方法24只Wistar大白鼠肝左叶种植Walker-256肿瘤后随机分为两组,肿瘤植入后第8d行肝动脉结扎和单纯开腹手术。术后第1、3、7、12d各处死3只大鼠,取出肝肿瘤,通过免疫组织化学染色检测缺氧诱导因子-1α(HIF-1α)及血管内皮生长因子(VEGF)表达,采用彩色图像分析系统计算平均光密度;SPSS软件作统计学分析。结果肝动脉结扎术后第1、3d,肝动脉结扎组的HIF-1α及VEGF表达显著高于剖腹对照组(P<0.05)。术后第7、12d,HIF-1α与VEGF表达两组间无显著性差异(P>0.05)。肝动脉结扎组中,HIF-1α与VEGF表达具有良好的相关性(r=0.60,P<0.05)。结论肝动脉阻断后缺氧微环境刺激肿瘤细胞通过HIF-1通路产生适应性改变。Objective To investigate the molecular biologic effect of transcatheter arterial embolization （TAE） on hepatic tumor in hypoxia. Materials and Methods 24 Wistar rats were randomly assigned into two groups： The HAL group and the control group. 8 days after Walker 256 carcinoma was implanted in the left medial lobes of the livers, hepatic artery ligation （HAL） and abdominal incision were performed. Each three rats were sacrificed 1, 3, 7, 12 day after treatment and tumor tissues were excised. Expression of hypoxia inducible factor la （HIF-1α） and vascular endothelial growth factor （VEGF） protein of tumors were examined using immunohistochemistry. The staining intensity of HIF-1α and VEGF were evaluated with a computer assisted image analyzer. The results were analyzed with SPSS software. Results 1, 3 d after treatment, the staining intensity of HIF-1α and VEGF were significantly higher in the HAL group than that in the control group （P 〈 0.05 ）. 7, 12 d after treatment, expression of HIF-1α and VEGF protein had no significant difference in the HAL group compared with the control group （ P 〉 0.05）. VEGF was well correlated to HIF-1α expression in the HAL group （ r = 0.60, P 〈 0.05 ）. Conclusion Tumor is adapted to hypoxic microenvironment through HIFI pathway after hepatic arterial blockage.

关 键 词：癌 肝细胞 肝动脉阻断 缺氧诱导因子 血管内皮生长因子 

分 类 号：R735.7[医药卫生—肿瘤]