检测胰液中K-ras基因突变对胰腺癌诊断的作用  

Role of K-ras gene mutation in pancreatic juice in the diagnosis of pancreatic carcinoma

作　　者：彭林[1] 王卫东[1] 陈盛[1] 

机构地区：[1]广东省人民医院肝胆胰外科,广州市510080

出　　处：《实用医学杂志》2006年第18期2109-2111,共3页The Journal of Practical Medicine

基　　金：广东省科技计划项目(编号:2002C31109)

摘　　要：目的:检测经逆行胰胆管造影(ERCP)过程中所获胰液中的K-ras基因突变。探讨其在胰腺癌诊断中的作用和意义。方法:收集ERCP过程中注射促胰泌素后的胰液,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法检测胰腺良、恶性疾病胰液上清和细胞中的K-ras基因突变。结果:胰腺癌病例的胰液上清液中K-ras基因突变检出率为60.0%(18/30),细胞中的检出率为76.7%(23/30)。胰腺良性疾病胰液细胞中有15.4%(2/13)的K-ras基因突变,上清液和其他非胰腺疾病对照组胰液中无K-ras基因突变检出。胰腺癌患者的胰液中检测到K-ras基因突变明显高于胰腺良性疾病患者和对照组(P<0.001)。K-ras基因突变检出率与肿瘤的部位及大小无明显关系。结论:胰液K-ras基因突变检出对于胰腺癌的诊断具有一定作用。Objective To explore the role of K-ras gene mutation in pancreatic juice in the diagnosis of pancreatic carcinoma, Methods The pancreatic juice was collected 100 minutes after injection of 100 U secretin during the procedure of ERCP, PCR-RFLP method was used to detect K-ras gene mutation in the supernate and cells from the pancreatic juice in malignant and benign pancreatic diseases. Results K-ras gene mutation in the supernate and cells from the pancreatic juice was confirmed in 60.0% and 76.7% of the patients with pancreatic carcinoma, respectively, Only 15.4% of K-ras gene mutation in cells from the pancreatic juice were detected in the patients with benign pancreatic diseases and no K-ras mutation in the supernate was found in those patients and the patients without pancreatic diseases. The K-ras mutation rate in the patients with pancreatic carcinoma was significantly higher than that in the patients with benign pancreatic diseases and the patients without pancreatic diseases （P 〈 0.001 ） and it had little correlation with the location and size of the tumor. Conclusion Detection of K-ras gene mutation plays a role in the diagnosis of pancreatic carcinoma.

关 键 词：胰腺肿瘤 胰液 K-RAS基因 

分 类 号：R735.9[医药卫生—肿瘤] R979.5[医药卫生—临床医学]