角膜移植术后肿瘤坏死因子-α受体Ⅰ含量的变化  

Experimental study on the change of TNF-α receptor Ⅰ after penetrating keratoplasty

作　　者：温淑梅 李贵仁[2] 康凤英[2] 

机构地区：[1]浙江中医学院第二附属医院眼科,杭州310007 [2]潍坊医学院附属医院眼科

出　　处：《临床眼科杂志》2006年第4期371-373,I0002,共4页Journal of Clinical Ophthalmology

摘　　要：目的检测角膜移植术后肿瘤坏死因子-α受体Ⅰ(tumor necrosis factor alpha receptorⅠ,TNFRⅠ)的含量变化并探讨其与角膜移植免疫排斥反应的关系。方法分别以鸡、兔为供受体建立异种异体穿透性角膜移植模型,对术后不同时间的供受体角膜行TNFRⅠ的免疫组织化学染色,并对其结果进行计算机图像分析,同时观察自体移植组(B)、异体移植CsA治疗组(C)及异种异体移植组(D)各项指标的变化。结果术后10天,D组TN-FRⅠ阳性细胞光密度(optical density,OD)值(0.23)及面积值(63.40μm2)高于B组(q=5.10,P<0.01)及C组(q=3.27,P<0.05);D组发生排斥反应时,OD值及面积值增加,而相应时间B组角膜TNFRⅠOD值及面积值减少,C组变化不明显。结论TNFRⅠ参与角膜移植免疫排斥反应,其含量变化与排斥反应的发生密切相关。Objective To determine the change of tumor necrosis factor-alpha receptor Ⅰ （TNFR Ⅰ ） content after penetrating keratoplasty and study the relation between TNFR Ⅰ and corneal graft rejection. Methods We used the chicken to rabbit model of penetrating heterograft. Immunohistochemical and image analysis of TNFR Ⅰ was performed on cemea in different time points of postoperation. The changes of corneal histopathy were also observed with microscope. Results At post-operative day 10, TNFR Ⅰ-positive ceils were detected at donor-recipient rim in every group. There was no significant difference between the B group and the C group, hut TNFR Ⅰ -positive ceils of TNFR Ⅰ of D group were more than other groups. The positive cells optical density and area of TNFR Ⅰ in D group increased significantly when the rejection happened. In the same time, we could ouly find little TNFR Ⅰ -positive ceils at donor - recipient rim in C group and B group, optical density and area of TNFR Ⅰ -positive cells in C group were higher than that in B group. Conclusions Tumor necrosis factor - alpha receptor Ⅰ played critical role in cemeal graft rejection. The change of TNFR Ⅰ content associated with the rejection.

关 键 词：肿瘤坏死因子-α 受体 角膜移植 排斥反应 

分 类 号：R779.65[医药卫生—眼科]