利用具有pH检测控制和补料功能的新型摇床研究利福霉素SV种子培养  

Studies on rifamycin SV seed cultivation with pH-controlled fed-batch shaker

作　　者：杜吉泉[1] 王军峰[1] 储炬[1] 王永红[1] 庄英萍[1] 张嗣良[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室国家生化工程技术研究中心,上海200237

出　　处：《中国抗生素杂志》2006年第8期462-465,487,共5页Chinese Journal of Antibiotics

基　　金：上海市科委重点科技攻关项目(034319220)

摘　　要：本文利用具有pH检测控制与补料功能的新型摇床,得到了利福霉素SV种子的最适接种量范围为6%～8%(V/V)及最适移种时间为对数生长期末期(判断标准为pH到达第二个低谷),并建立了预测菌体浓度的数学模型。检验证明此模型能够较准确地预测利福霉素SV产生菌在对数生长期及移种时(对数生长期末期)的菌浓,并可用于预测在一定接种量下达到预定菌浓所需的培养时间。本模型在适宜的接种量范围内准确度较高,但随接种量的偏离程度的增加准确度有所减小;另外本模型只在培养基成份、培养环境等条件不变时适用,否则模型参数需重新估计。The process of vegetative seed cultivation was investigated with pH-controlled fed-batch shaker （pH-shaker）. The optimized time window for seed transferring was the second valley of the pH profile and the opti- mized inoeulum size was 6% - 8% （ V/ V）, Furthermore, a mathematical model for predicting the packed myeelial volume of the vegetative seed process was developed, which was proved suitable for logarithmic phase of vegetative seed growth. The accuracy of the model was is high under appropriate inoeulum size but it will descend following with the inappropriate inoculation. In addition, the model is suitable only when the inoculation conditions such as medium, temperature and rotation are invariable, otherwise the parameters of the model should be recalculated.

关 键 词：pH检测控制 利福霉素SV 培养过程优化 菌浓预测 数学模型 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]