-80℃冻存外周血造血干细胞的应用研究  

Study on the Freezing Procedure to Store Peripheral Blood Stem Cells at -80℃

作　　者：袁成录 史春雷 王玲 李颖 黄梅娟 王媛媛 高岱清 

机构地区：[1]山东省青岛市中心医院内科,山东青岛266042

出　　处：《河北医学》2006年第9期864-866,共3页Hebei Medicine

摘　　要：目的:探求一种操作简便实用、造血干细胞回收率和存活率高、成本低的外周血造血干细胞的冻存方法。方法:CP-1和5%白蛋白作保护剂在?80℃条件下直接冻存,并检测冻存前后外周血干细胞的活性,单个核细胞(MNC)和CFU-GM、CD34+细胞的回收率。结果:冻存前后外周血干细胞活性,单个核细胞(MNC)和CFU-GM、CD34+细胞的回收率无明显差异(P>0.05),输注后造血均重建。结论:CP-1和5%白蛋白作保护剂在?80℃条件下直接冻存干细胞是一种简便、可靠、安全、成本低的外周血干细胞冻存方法,具有广泛的临床推广价值。Objective： To seek a convenient, practical freezing procedure with high recovery rate and survival rate as well as low cost to store peripheral blood （PB） stem cells. Method：The PB stem cells were froze at - 80℃ direcdy with CP - 1 and 5% albumin as protective agents. The activity of PB stem cells and recycle rate of mononuclear cell （ MNC ）, CFU - GM and CD34^＋ cells before and after freezing were detected. Result：The activity of PB stem cells and recovery rate of MNC, CFU -GM and CD34^＋ cells have no significant differences before and after freezing （ P 〉 0.05 ）. Hemopoiesis could be reconstructed after infusion all. Conclusion：Freezing stem ceils directly at - 80℃ by using CP - 1 and 5% albumin as protective agents is a convenient, reliable, safe and low cost procedure to store PB stem cells, which has the possibility to be used in clinical widely.

关 键 词：造血干细胞移植 外周血干细胞 冻存方法 

分 类 号：R733[医药卫生—肿瘤]