脂肪酶催化合成环十五内脂的酸碱效应  

Acid-base Effect of Cyclopentadecanolide Synthesis by Lipase Catalysis

作　　者：黄宏妙[1] 沈芳[1] 刘雄民[1] 马丽[1] 梁静娟[1] 

机构地区：[1]广西大学化学化工学院,广西南宁530004

出　　处：《化学世界》2006年第8期497-499,共3页Chemical World

基　　金：教育部科学技术重点项目资助(204110);广西自然科学基金资助项目(桂科自0135013)

摘　　要：以15-羟基十五烷酸甲酯为底物，进行了脂肪酶催化合成环十五内酯研究，考察了酶活性与pH值的关系，探讨了酶粉pH值记忆和有机相酸碱性对内酯化反应的影响。结果表明，pH=6～7时脂肪酶的水解活力最高，pH=6～6．5时15-羟基十五烷酸甲酯转化环十五内酯的转化率最高，添加有机酸碱对内酯化反应产生不利影响。The synthesis of cyclopentadecanolide by lipase catalysis was studied, in which methyl 15-hydroxypentadecanate was used as the substrate. The relation between activity of lipase and pH was investigated, and the effects of factors such as pH-imprinting and acid-base strength of the organic phase on lactonization were discussed. The results showed that the activity of lipase reached maximum when the pH was 6 - 7, and the conversion of cyclopentadecanolide from methyl 15-hydroxy-pentadecanate was the highes when the pH was 6- 6.5 ,but adding organic acid and alkali was unfavorable for the lactonization reaction.

关 键 词：脂肪酶 环十五内酯 15-羟基十五烷酸甲酯 

分 类 号：O643[理学—物理化学] Q556[理学—化学]