乙酰肝素酶mRNA、层粘连蛋白及其受体在大肠癌中的表达  

Expression of heparanase mRNA,laminin and laminin receptor in colorectal carcinoma

作　　者：牛保华[1] 皇甫超申[1] 赵卫 

机构地区：[1]河南大学医学院神经生物学研究所 [2]开封市第一人民医院病理科

出　　处：《山西医科大学学报》2006年第6期589-591,共3页Journal of Shanxi Medical University

基　　金：河南省教育厅科研基金资助项目(20023100012)

摘　　要：目的探讨大肠癌中乙酰肝素酶及相关基膜蛋白的表达在大肠癌转移中的作用。方法应用原位杂交和免疫组化方法检测乙酰肝素酶mRNA、层粘连蛋白(LN)及受体(LR)在90例大肠癌组织中的定位及表达,探讨其在大肠癌转移中的作用。结果90例大肠癌组织中,乙酰肝素酶mRNA、LN及LR阳性表达率分别为51.11%,37.78%和46.67%。52例大肠癌伴淋巴结转移者,其原发灶内乙酰肝素酶mRNA、LR阳性表达率分别为63.46%和61.53%,明显高于无转移组(28.94%,26.31%),LN表达(26.92%)明显低于无转移组(52.63%),有显著差异(P<0.05)。乙酰肝素酶mRNA、LN、LR表达与大肠癌淋巴结转移有关(P<0.05)。结论乙酰肝素酶、LN和LR可能在大肠癌的生长、浸润和转移中起重要作用。Objective To study the, role of heparanase mRNA, lamimin and lamimin receptor in colorectal carcinoma. Methods The expression of heparanase mRNA was detected by ISH and the lamimin and lamimin receptor by immunohistochemistry in 90 cases of colorectal carcinoma. Results.The expression rate of heparanase mRNA,LN and LR in these cases of colorectal carcinoma were 48.88%, 37.78 % and 46.67 %, respectively. The increased expression of heparanase mRNA, LN and LR and the decreased expression of LN were observed in 52 cases of colorectal carcinoma with lymph node metastasis compared to those without metastasis （P〈 0.05）. The correlation betwcen heparanase mRNA, LN and LR expressions were related with lymph node metastasis （P 〈0. 01 ）. Conclusion Heparanase, LN and LR may play an imtportant role in the growth, invasion and metastasis of colorectal carcinoma.

关 键 词：乙酰肝素酶 层粘连赁白 受体 层粘连蛋白 结直肠肿瘤 肿瘤转移 

分 类 号：R735.34[医药卫生—肿瘤]