The Relationship Between Senescence and Ca^(2+)-ATPase Activity of Microsomal Membrane and Lipid Peroxidation in Harvested Peach Fruit  

作　　者：GUAN Jun-feng FAN Xiu-cai DOU Shi-juan ZHANG Ji-shu LI Guang-min 

机构地区：[1]Institute Of Genetics and Physiology, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050051, P.R.China [2]Zhengzhou Fruit Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450002, P.R.China [3]Northwest A＆F University, Yangling 712100, P.R.China

出　　处：《Agricultural Sciences in China》2006年第8期609-614,共6页中国农业科学（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China （30270933）;Natural Science Foundation of Hebei Province, China （303600）.

摘　　要：Peach fruit easily soften and have a short storage time at normal temperature. In this study, peach fruit （Prunus persica sieb et Zucc cv. Yingqing） were picked and stored at 25 and 4℃ to investigate the senescence in correlation with Ca^2＋- ATPase activity of microsomal membrane and lipid peroxidation during ripening and senescence. In comparison with that stored at 25~C, the fruit stored at 4℃ exhibited a higher flesh firmness, lower respiration rate, and generated the late bigger peak value of Ca^2＋-ATPase activity as well as maintained the higher activity of the enzyme. Meanwhile, the lower levels of super oxygen radical （O2^-.） production and content of malondialdehyde （MDA）, a product of membrane lipid peroxidation were observed. Sodium orthovanadate （SO） and erythrosin B （EB）, as Ca^2＋-ATPase inhibitors, could stimulate the respiration rate. The results suggested that the slower senescence rate of peach fruit was closely related to the higher peak value and longer duration of Ca^2＋-ATPase activity in microsomal membrane, with the slighter membrane lipid peroxidation and lower O2^-. production rate.Peach fruit easily soften and have a short storage time at normal temperature. In this study, peach fruit （Prunus persica sieb et Zucc cv. Yingqing） were picked and stored at 25 and 4℃ to investigate the senescence in correlation with Ca^2＋- ATPase activity of microsomal membrane and lipid peroxidation during ripening and senescence. In comparison with that stored at 25~C, the fruit stored at 4℃ exhibited a higher flesh firmness, lower respiration rate, and generated the late bigger peak value of Ca^2＋-ATPase activity as well as maintained the higher activity of the enzyme. Meanwhile, the lower levels of super oxygen radical （O2^-.） production and content of malondialdehyde （MDA）, a product of membrane lipid peroxidation were observed. Sodium orthovanadate （SO） and erythrosin B （EB）, as Ca^2＋-ATPase inhibitors, could stimulate the respiration rate. The results suggested that the slower senescence rate of peach fruit was closely related to the higher peak value and longer duration of Ca^2＋-ATPase activity in microsomal membrane, with the slighter membrane lipid peroxidation and lower O2^-. production rate.

关 键 词：peach fruit Ca^2＋-ATPase active oxygen lipid peroxidation SENESCENCE 

分 类 号：S662.1[农业科学—果树学]