hMSH2蛋白和P53蛋白表达在胃癌发病中的意义  

Significance of gene product expressions of hMSH2 and P53 in gastric carcinogenesis

作　　者：刘飞飞[1] 李梅[1] 王朝晖[1] 吕申[1] 

机构地区：[1]大连医科大学第二临床学院实验中心,辽宁大连116027

出　　处：《大连医科大学学报》2006年第4期286-287,298,共3页Journal of Dalian Medical University

基　　金：中国科学院知识创新工程领域前沿基金项目"国人胃癌遗传背景的研究"(No.DICPkaaaabc)的一分支

摘　　要：[目的]探讨DNA错配修复基因hMSH2蛋白、抑癌基因P53蛋白表达在胃癌发病中的意义。[方法]采用免疫组织化学技术(S-P法)检测63例胃癌组织及32例胃炎组织hMSH2、P53的表达。[结果]胃癌组hMSH2蛋白阳性表达率(79.37%)明显高于胃炎组(53.13%)(P<0.05);胃癌组P53蛋白阳性表达率(88.89%)明显高于胃炎组(21.88%)(P<0.05)。hMSH2蛋白在高中分化腺癌、低分化腺癌及黏液癌的阳性表达率分别为73.68%、82.76%和80.00%,P53蛋白阳性表达率分别为100.00%、89.66%和73.33%,两者阳性表达率与胃癌分化程度均无显著性差异(P>0.05)。hMSH2蛋白和P53蛋白表达呈正相关。[结论]DNA错配修复基因hMSH2蛋白及抑癌基因P53蛋白的异常表达可能参与胃癌发生机制。[ Objective] To study the relationship of expressions of gene product hMSH2, P53 to gastric carcinogenesis. [ Method] Immunohistochemical technique was used to detect hMSH2 and P53 protein expressions in 63 cases of gastric carcinomas and 32 cases of gastritis. [ Results] The positive rate of hMSH2 in gastric carcinoma being 79.37 % （50/63） was significantly higher than that in gastritis being 53.13% （17/32） （ P 〈 0.05） ; the positive rate of P53 in gastric carcinoma being 88.89% （56/63） was significantly higher than that in gastritis being 21.88%（7/32） （ P 〈 0.05）. The positive rates of hMSH2 in well medial differentiation, poor differentiation and mucoid carcinoma were 73.68%, 82.76% and 80.00% respectively（ P 〉 0.05）, and the positive rates of P53 were 100.00%, 89.66% and 73.33% respectively（P 〉 0.05）. The expression of hMSH2 was positively correlated to the expression of P53 in gastric mucosa including carcinoma and gastritis （ r = 0.223, P 〈 0.05）. [ Conclusion] The abnormal expressions of mismatch repair gene hMSH2 and tumor suppressor gene P53 may play some roles in the gastric carcinogenesis.

关 键 词：错配修复基因 HMSH2 抑癌基因 p53 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]