香菇多糖提取纯化研究  

Study on Extraction and Purification of Lentinan

作　　者：卢成英[1] 李国章[1] 黄早成[1] 郭晓辉[1] 曹庸[1] 

机构地区：[1]湖南省林产化工工程重点实验室吉首大学张家界校区,湖南张家界427000

出　　处：《中国林副特产》2006年第4期5-7,共3页Forest By-product and Speciality in China

摘　　要：用0.2mol/L三氯乙酸、0.2mol/L氢氧化钠、1.0mol/L乙酸、蒸馏水为提取溶剂,结合超声波处理等方法从香菇子实体中提取香菇多糖,提取液经甲醇除杂、沉淀处理得粗多糖。粗多糖用CTAB络合沉淀,沉淀物乙酸溶解后取上清再用甲醇沉淀,沉淀物冻干得纯化多糖。分析检测结果显示干香菇三氯乙酸提取的纯化多糖的含量高达93.1%、提取得率达0.324%。薄层分析表明,纯化香菇多糖由葡萄糖、甘露糖、半乳糖、木糖组成。纯化多糖用Sephadex G-100凝胶层析,洗脱液经苯酚-硫酸法检测为单一吸收峰,红外光谱显示其具有多糖类的特征吸收峰。Usmg 0.2mol/L CCl3COOH, 0. 4 mol/L NaOH, 1.0 mol/L CH3COOH, water as solvent , polysaccharide was extracted from Lentinus edodes. After 50 % methyl alcohol eliminate impurity and 75 % methyl alcohol precipitate Lentinan crude. Then drying the Lentinan crude , using water to dissolve it, and adding 0.15mol/L CTAB of same volume to precipitate Lentinan, precipitate was added 2mol/L acetic acid to dissolve Lentinan and got rid of the infusible thing. Using 50% methyl alcohol to eliminate impurity in the filtrate, then adding 75 % methyl alcohol to precipitate Lentinan again. Purity of the Lentinan extracted by 0. 2 mol/L of CCl3COOH is up to 93.1%, the extraction rate is 3.24‰. TLC showed that the Lentinan contain glucose, mannose,galactose and xylose, sephadex-100 column chromatography and vitriol- phenol method suggested that Lentinan has one peak, IR spectrum suggested that Lentinan has a chart, which looks the same to that of amylose.

关 键 词：香菇多糖 提取 纯化 

分 类 号：S646.12[农业科学—蔬菜学]