吡格列酮对高糖高胰岛素诱导的肥大心肌细胞中CT-1的影响  

Effects of pioglitazone on cardiotrophin-1 expression in hypertrophic cardiomyocytes induced by high glucose and insulin

作　　者：刘建军[1] 李振中[1] 鞠瑛[2] 李永刚[1] 邵峰[3] 刘真[1] 王怀经[1] 邢子英[1] 

机构地区：[1]山东大学医学院解剖学教研室,山东济南250012 [2]山东大学医学院免疫学研究所,山东济南250012 [3]葛洲坝中心医院内科,湖北宜昌443002

出　　处：《山东大学学报（医学版）》2006年第8期765-769,共5页Journal of Shandong University:Health Sciences

摘　　要：目的:观察心肌营养素-1(CT-1)在高糖高胰岛素诱导的肥大心肌细胞中的表达,以及吡格列酮对其表达的影响。方法:体外培养乳鼠心肌细胞,分组给药后,检测心肌细胞肥大的指标:心肌细胞的表面积(图像分析法)、蛋白含量(考马斯亮蓝法)、心房利钠因子(ANF)mRNA的表达(RT-PCR法);同时用半定量RT-PCR检测CT-1 mRNA的表达。结果:高糖高胰岛素组心肌细胞表面积、蛋白含量、ANFmRNA表达以及CT-1 mRNA水平均升高(P<0.01),而吡格列酮治疗组降低(P<0.05)。结论:高糖高胰岛素诱导的肥大心肌中CT-1表达上升,而吡格列酮能抑制其表达,推测CT-1可能参与了高糖高胰岛素所引起的心肌肥大的发生,而吡格列酮抑制心肌肥大的作用可能是通过抑制CT-1介导的。Objective： To study the expression of cardiotrophin-1 （CT-1） in hypertrophic cardiac myocytes induced by high glucose and insulin, and the effect of pioglitazone on the expression of cardiotrophin-1 （CT-1）. Methods： The cardiac myocytes were cultured in vitro as a model. Cellular surface, protein content and atrial natriuretic factor mRNA were measured as the cellular hypearophic parameters. Meanwhile, the expression of cardiotrophin-1 mRNA was assayed with semiquantitative RT-PCR. Results： After treatment with high glucose and insulin, cellular surface, protein content and expression of atrial natriuretic factor mRNA increased significantly （ P 〈 0.01 ）. Pioglitazone inhibited all these above effects effectively （ P 〈 0.05）. Condusion： The results suggest that cardiotrophi-1 is involved in the development of myocardial hypertrophy induced by high glucose and insulin. And pioglitazone may inhibit myocardial hypertrophy by restraining the expression of cardiotrophin-1.

关 键 词：吡格列酮 高糖 高胰岛素 心肌肥大 心肌营养素-1 大鼠 Wistar 

分 类 号：R-542.205[医药卫生] R-332