几个因素对木霉菌株T-33产生厚垣孢子的影响  

Effects of Several Factors on the Chlamydospores Production of Trichoderma aureoviride T-33

作　　者：邹勇[1] 文成敬[1] 唐贵群[1] 李宁[1] 

机构地区：[1]四川农业大学植物保护系,雅安625014

出　　处：《微生物学通报》2006年第4期43-47,共5页Microbiology China

基　　金：四川省农业厅新农药试验项目基金(No.1236)

摘　　要：研究了几个因素对黄绿木霉(Trichoderma aureoviride)T-33菌株发酵产生厚垣孢子的影响,并在此基础上进行正交试验,得到了主要影响因子的较好组合。实验表明,适合T-33产生厚垣孢子的最佳单因素条件分别是:燕麦粉培养液,30℃,pH4·0,120r/min,24h振荡培养,采用250mL三角瓶培养时,装入培养液的最适量为80mL/瓶;正交试验表明,影响产孢的主要因子温度、pH和振荡速度的最佳组合为30℃、pH4·0、140r/min。在这样的条件下,可获得的厚垣孢子量为3·37×107个/mL。The effects of several factors on the chlamydcepores production of Trichoderma aureoviride T-33 during the fermentation were researched. Based on the results above, the orthogonal test was made to screen out the best prescription. The results showed that, the best single-factor conditions for the chlamydespores production of T. aureoviride T-33 were, liquid culture of oat powder, 30℃, pH4. 0, 120r/min, 24 hours oscillate incubating as well as liquid culture volume of 80mL/bottle when the 250mL size triangle bottle was used. The result of orthogonal test showed that, the best prescription for temperature, pH and oscillating speed was 30℃, pH4. 0 and 140r/min. 3.37 × 10^7 spore/mL chlamydespores were obtained at this combined condition.

关 键 词：黄绿木霉 厚垣孢子 发酵条件 正交试验 

分 类 号：S476[农业科学—农业昆虫与害虫防治]