藤黄微球菌海藻糖生物合成基因的克隆与鉴定  

Cloning and Analysis of Genes Correlated to Trehalose Biosynthesis from Micrococcus luteus

作　　者：黄雪锋[1] 欧阳立明[1] 吴海珍[1] 叶江[1] 张惠展[1] 袁勤生[1] 

机构地区：[1]华东理工大学生物工程学院,上海200237

出　　处：《微生物学通报》2006年第4期74-79,共6页Microbiology China

摘　　要：首次从一株能利用淀粉产生海藻糖的藤黄微球菌中克隆了海藻糖生物合成基因。采用PCR方法结合非随机鸟枪法得到藤黄微球菌中低聚麦芽糖苷基海藻糖合成酶(MTSase)基因(MtreY)的全序列及低聚麦芽糖苷基海藻糖水解酶(MTHase)基因(MtreZ)的部分序列,其中MtreY共有2,370个碱基,编码789个氨基酸,表达产物分子量为86·7kD。同源性分析表明,与已报道的MTSase和α-淀粉酶家族成员具有相同的保守模体。将MtreY基因在大肠杆菌JM83中表达,证明表达产物具有预期的酶活性。Genes related to trehalose biosynthesis from a bacterial strain Micrococcus luleus which can convert pardally hydrolyzed starch into trehalose were cloned. Full sequence of gone （MtreY） encoding trehalose maltooligosyl trehalose synthese （MTSase） and partial sequence of gene （MtreZ） encoding maltooligosyl trehalose trehalohydrolase （MTHese） were got using PCR combined non-random shotgun method. Sequence analysis of MtreY predicts a 2, 370bp open reading frame encoding a protein of 790 amino acids with a predicted molecular weight of 86734 Da. Homologous analysis shows that this new gene has the same conservative motifs with a-amylase family enzymes. The MtreY gene was expressed in E. coli, and the expression product has the anticipetive enzyme activity.

关 键 词：藤黄微球菌 海藻糖 低聚麦芽糖苷基海藻糖合成酶(MTSase) 低聚麦芽糖苷基海藻糖水解酶(MTHase) 同源性分析 

分 类 号：Q933[生物学—微生物学] Q78