蛋黄中抗嗜水气单胞菌IgY的提取和纯化  

Extraction and Purification of IgY against Aeromonas Hydrophila from Egg Yolk

作　　者：李肖梁[1] 钱娅 祝春蕾 方维焕[1] 

机构地区：[1]浙江大学动物预防医学研究所,浙江杭州310029 [2]杭州联合肉类集团,浙江杭州310004 [3]浙江明珠动物保健品有限公司,浙江杭州312000

出　　处：《中国家禽》2006年第15期10-12,15,共4页China Poultry

基　　金：杭州市科技局重点项目(20051322B33);湖州市农业攻关计划(2005GN05);嘉兴市星火计划项目

摘　　要：通过对蛋黄中抗嗜水气单胞菌IgY的提取,分别进行了两种粗提方法(水稀释法、Pectin法)和纯化方法(硫酸铵法、PEG6000法)的比较。结果显示,当蛋黄液经pH5.0蒸馏水10倍稀释粗提后,上清液中脂肪和IgY残余率分别为32.69%和68.34%,效果较好。采用12%(w/v)PEG6000、33%(v/v)饱和硫酸铵纯化IgY,其免疫活性残余率分别为63.5%和64.1%。SDS-PAGE显示,水稀释法和Pectin法提取的上清液中杂蛋白减少,硫酸铵和PEG6000纯化后的上清液中几乎无其他杂带。研究表明,水稀释法和Pectin法提取IgY具有简便、高效和无污染的特点,硫酸铵和PEG6000适合纯化IgY。Different methods of extracting（water dilution and Pectin） and purifying（saturated ammonium sulfate and PEG 6 000） were conducted to get IgY against Aeromonos Hydrophila. The results showed that remaining ratio of fat and IgY were 32.69% and 68.34% in suspension of egg yolk extracted with 10-floder diluted water at pH 5.0 and the effect was better. Remaining immunocompetence ratio of IgY was 63.5% and 64.1% purified by 12% PEG 6 000（w/v） and 33% saturated ammonium sulfate （v/v） respectively. SDS-PAGE indicated the amount of non-IgY protein significantly decreased by water dilution and Pectin methods,and there were no other proteins except IgY in supernatants with PEG 6 000 and saturated ammonium sulfate as precipitants. Thin paper showed water dilution and Pectin methods provided a simple,rapid and efficient means of extracting crude IgY,and PEG 6 000 and saturated ammonium sulfate were suitable to purify IgY in suspension.

关 键 词：IGY 提取 纯化 蛋黄 嗜水气单胞菌 

分 类 号：S941.42[农业科学—水产养殖] TS202.3[农业科学—水产科学]