沙门氏菌单克隆抗体研究 Ⅰ.杂交瘤细胞系的建立  

Studies on Monoclonal Antibodies against Salmonella Antig-ens I.Establishements of Hybridoma Cell Lines

作　　者：蔡玲民 吴采菲 赵红[1] 陈翠萍[1] 刘新铭[1] 高鸿瑞[1] 袁皓加[1] 张河战[2] 马越[2] 辜清吾[2] 

机构地区：[1]卫生部兰州生物制品研究所,兰州730046 [2]中国药品生物制品检定所,北京100050

出　　处：《单克隆抗体通讯》1990年第2期22-30,共9页

摘　　要：用沙门氏菌O,H,Vi抗原免疫BALB/c小鼠,采用细胞融合技术,获得分泌抗沙门氏茵0-2,4,7,8,9,3,10,11和H-a,b,c,d,i及Vi抗原的单克隆抗体杂交瘤细胞系29株。所产生的抗体17株属IgM,7株为IgG3,3株为IgG1,1株为IgG_(2a),1株未测;轻链除3株为λ链外,其余为κ链。将杂交瘤细胞所诱生的腹水,组成一套(11种)沙门氏菌诊断用单克隆抗体试剂,经用沙门氏菌属和其它肠道菌属的代表菌株作鉴定,证明具有良好的特异性和敏感性、可供初步诊断伤寒,副伤寒和鼠伤寒用。与目前常规生产的家兔诊断血清相比,具有简化生产过程,节约家兔与培养基及省时省力等优点,值得在生产上和使用上推广。BALB/c mice were immunized with Salmonella strains containing different. Hf and Vi antigens or purified If and V i antigens. The spleen cells were fused with myeloma cells Sp2/0. 29 strains o f hybridoima cell secret ing monolonal antibodies againsl Salmonella antigen 0:2,4, 7, 8, 9, 3, 10, 11, H:a, b, c, d, i and Vi respectively, were obtained by screening. Among the 29 strains, 17 belonged to IgM, 7 lgG3, 3 IgC1, 1 IgG2a, but 1 was not determined. 3 of them were λ chain and the rest,k chain. McAb asciles were determined with represcnlative strains of Salmonella and other enlerobaeteria. Most of them possessed high specificity and sensitivity. A kit of dia-gnoslic reagents of Salmonella MeAbs(11 kinds)was produced for the use of preliminary diagnosis of S. typhi, S. paratyphi and S. typhimurium. The production processes of I he Salmonella McAbs were more convenient than that of the traditional products. Thus it would benefit in the use of them.

关 键 词：沙门氏菌 杂交瘤 单克隆抗体 

分 类 号：R392－33[医药卫生—免疫学]