蔗渣半纤维素不同组分的水解  

Hydrolysis of Different Hemicelluloses Extracted from Bagasse

作　　者：陈鹏[1] 林鹿[1] 徐丽丽[1] 杨柳[1] 雷晓春[1] 何北海[1] 付时雨[1] 孙润仓[1] 

机构地区：[1]华南理工大学资源科学与工程系制浆造纸工程国家重点实验室,广州510640

出　　处：《造纸科学与技术》2006年第4期18-21,共4页Paper Science & Technology

摘　　要：对蔗渣半纤维素不同组分用酸或碱水解、酶解、酶解后再酸/碱水解的不同方法进行水解,利用高效液相色谱(HPLC)分析水解液中单糖的组成,探讨半纤维素不同组分的水解过程与产物组成的关系。实验结果显示对半纤维素A用半纤维素酶(酶活400 IU/g)法水解比用20%NaOH水解效果好,木糖得率达34.87%;而对于半纤维素B,采用酶法水解比7%H2SO4水解得率相差较大,采用酶解后7%H2SO4水解可得得率为23.60%。Different hemicelluloses extracted from bagasse were hydrolyzed by acid or alkali hydrolysis, enzymolysis, enzymolysis after acid or alkali hydrolysis. The compositions of monose in hydrolyzate were analyzed by HPLC, and the relation between different hydrolyzing conditions and the composition of the products was set up. The results of experiment showed that to hemicellulose A, the enzymolysis with hemicellulase （enzymatic activity 400IU/g） got a higher productivity than alkali hydrolysis, the yield of xylose was 34.87% , but to hemicellulose B, the productivity of enzymolysis was lower than that of acid hydrolysis, the yield of xylose after hemicellulase-acid hydrolysis was 23.60%.

关 键 词：蔗渣 半纤维素 半纤维素酶 水解 高效液相色谱 

分 类 号：TS749.3[轻工技术与工程—制浆造纸工程]