S180肉瘤细胞腹水涂片的三种染色方法比较  

Comparison of Three Staining Techniques for S180 Ascites Smear Observation

作　　者：张东辉[1] 许永华[1] 许琴[1] 李建瑛[1] 张培谊[2] 祝湘[1] 是文辉[1] 殷学军[1] 

机构地区：[1]兰州军区乌鲁木齐总医院动物实验科,乌鲁木齐8300000 [2]兰州军区乌鲁木齐总医院病理科,乌鲁木齐830000

出　　处：《中国比较医学杂志》2006年第8期469-471,F0002,共4页Chinese Journal of Comparative Medicine

摘　　要：目的比较3种不同的染色方法,选择既简单又能客观反映S180肉瘤细胞特征的染色方法。方法使用HE染色法、巴氏染色法及快速染色法对S180肉瘤细胞腹水涂片进行观察。结果快速染色法操作简便;细胞结构最大限度保持原状;细胞质、细胞核、核仁之间色差较大,易于区别,能够最大限度反应细胞原有特征。结论在观察S180肉瘤细胞腹水涂片时,用快速染色法优于HE染色法和巴氏染色法。Objective Three different staining techniques were used to visualize S180 ascites smear morphology. The results were compared to find a best and simple staining technique showing most clear cytological characteristics. Method S180 ascites smear was stained by H.E. staining, Pap staining and fast staining, and the cell morphology was observed and compared under microscope. Results Among the 3 staining methods, the fast staining was most simple to operate and revealed cellular structures best. There was a best contrast among cytoplasm, nucleus and nucleolus in the smears stained by the fast staining, and the cell structures can be distinguished most clearly. Conclusion The fast staining is better than HE staining and Pap staining in S180 Aseites smear observation.

关 键 词：小鼠 肉瘤180 涂片 腹水 HE染色法 巴氏染色法 快速染色法 

分 类 号：R-331[医药卫生]