白腐真菌处理硝基苯化合物的机理初探  

Study on Mechanism of Degradation of Nitrobenzene Compounds by White Rot Fungi

作　　者：徐美红[1] 张学才[1] 李丽[1] 杨忠连[1] 陈明强[1] 刘玉林[1] 

机构地区：[1]安徽理工大学化学工程系,安徽淮南232001

出　　处：《化学与生物工程》2006年第8期40-42,共3页Chemistry & Bioengineering

基　　金：安徽省教育厅自然科学基金资助项目(2006kj111B)

摘　　要：以白腐真菌锰过氧化物酶(MnP)为主导酶处理硝基苯化合物,并研究了其独特的降解机理。通过测定不同浓度硝基苯溶液的吸光度,确定了硝基苯溶液标准曲线。用莫诺特(Monod)方程对实验数据进行模拟,应用Lineweav-er-Burk法进行数学回归分析。结果表明白腐真菌处理硝基苯化合物符合Monod提出的一级不可逆动力学模型,通过数学处理的方法确定其动力学特征参数vm=0.8325mg.L-1.min-1、kc=0.7525mg.L-1。Using white rot fungi Mn-dependent peroxidase as mare enzyme,the nitrobenzene compounas are degraded. And the unique degradation mechanism is studied. The standard curve of nitrobenzene solution is determined through the measurement of the absorbance with different nitrobenzene＇s concentration. The experimental data are modeled with Monod equation and are mathematical regression analyzed by Lineweaver-Burk method. The results indicate that the degradation of nitrobenzene compounds by white rot fungi is conformed to the first order irreversible dynamic equation of Monod model. By mathematic treatment,the dynamic character- istic parameters vm and k, are confirmed as 0. 8325 mg . L^-1. min^-1 and 0. 7525 mg . L^-1 respectively.

关 键 词：白腐真菌 锰过氧化物酶 硝基苯化合物 

分 类 号：TQ925[轻工技术与工程—发酵工程] Q814[生物学—生物工程]