4-1BB在人外周血γδT细胞活化中的协同刺激作用  

Costimulatory role of 4-1BB in the activation of human peripheral blood γδT cells

作　　者：吴俊英[1] 钱峰[1] 吕合作[1] 王伟[1] 

机构地区：[1]蚌埠医学院免疫学教研室,蚌埠233003

出　　处：《现代免疫学》2006年第4期327-330,共4页Current Immunology

基　　金：安徽省教育厅自然科学研究计划项目(2004kj280)

摘　　要：采用结核杆菌(Mtb)低分子多肽刺激人外周血单个核细胞,流式细胞术(FCM)检测不同活化时相γδT细胞膜表面4-1BB分子的表达;用阻断型4-1BB配体(4-1BBL)单抗阻断4-1BB/4-1BBL信号,FCM检测γδT细胞的增殖比率和细胞内产生IFN-γ的情况,同时与阻断CD28/B7-1信号相比较。结果显示,静止的γδT细胞膜表面不表达4-1BB分子,Mtb抗原刺激后6 h,4-1BB即有明显表达(29.71%),48 h达到高峰(49.79%);与未阻断组相比,阻断4-1BB/4-1BBL信号,γδT细胞的增殖效应和细胞内IFN-γ的产生均明显下降(P<0.01),与CD28/B7-1信号阻断组相比,差异无显著性(P>0.05)。提示4-1BB/4-1BBL信号同CD28/B7-1信号一样,可为γδT细胞活化提供协同刺激作用。Peripheral blood mononuclear cells （PBMC） were stimulated in vitro by a low-molecular peptide antigen of Mycobacterium tuberculosis （Mtb-Ag） expression of 4-1BB molecule on γδT cells was measured by flow cytometry （FCM） at different times after stimulation. By using the anti-4-1BB ligand （4-1BBL） monoclonal antibody to block the co-stimulatory signal of 4-1BB/4-1BBL interaction, the proliferative rate and the intracellular production of IFN-γ of γδT cells were detected by FCM. The experimental results showed that the 4-1BB molecule was expressed only on the activated γδT cells, and the surface expression of this molecule reached its peak at 48 hours after stimulation with Mtb-Ag. Meanwhile, the proliferative rate and the intracellular IFN-γ of γδT cells are obviously decreased after blockage of the costimulatory signal induced by 4-1BB/4-1BBL interaction. These results suggest that the costimulatory signal induced by the 4-1BB/4-1 BBL interaction may play an important role in the activation and proliferation of γδT cells.

关 键 词：4-1BB ΓΔT细胞 活化 增殖 

分 类 号：R392[医药卫生—免疫学]