A novel genome-wide full-length kinesin prediction analysis reveals additional mammalian kinesins  

作　　者：XUE Yu LIU Dan FU Chuanhai DOU Zhen ZHOU Qing YAO Xuebiao 

机构地区：[1]Laboratory of Cellular Dynamics, University of Science & Technology of China/Hefei National Laboratory, Hefei 230027, China [2]College of Agriculture, Zhejiang University, Hangzhou 310003, China

出　　处：《Chinese Science Bulletin》2006年第15期1836-1847,共12页

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.39925018,30121001,30270293&90508002);the Chinese Academy of Sciences(Grant No.KSCX2-2-01);the Chinese 973 Project(Grant No.2002CB713700);the Chinese 863 Project(Grant No.2001AA215331);Chinese Minister of Education(Grant No.20020358051);American Cancer Society(Grant No.RPG-99-173-01).

摘　　要：Kinesin superfamily of microtubule- based motor orchestrates a variety of cellular proc- esses. Recent availability of mammalian genomes has enabled analyses of kinesins on the whole ge- nome. Here we present a novel full-length kinesin prediction program (FKPP) for mammalian kinesin gene discovery based on a comparative genomics approach. Contrary to previous predictions of 94 kinesins, we identify a total of 134 potentially kinesin genes from mammalian genomes, including 45 from mouse, 45 from rat and 44 from human. In addition, FKPP synthesizes 25 potentially full-length mam- malian kinesins based on the partial sequences in the database. Surprisingly, FKPP reveals that full-length human CENP-E contains 2701 aa rather than 2663 aa in the database. Experimentation using sequence specific antibody and cDNA sequencing of human CENP-E validates the accuracy of FKPP. Given the remarkable computing efficiency and accuracy of FKPP, we reclassify the mammalian kinesin super- family. Since current databases contain many in- complete sequences, FKPP may provide a novel approach for molecular delineation of kinesins and other protein families.Kinesin superfamily of microtubulebased motor orchestrates a variety of cellular processes. Recent availability of mammalian genomes has enabled analyses of kinesins on the whole genome. Here we present a novel full-length kinesin prediction program （FKPP） for mammalian kinesin gene discovery based on a comparative genomics approach. Contrary to previous predictions of 94 kinesins, we identify a total of 134 potentially kinesin genes from mammalian genomes, including 45 from mouse, 45 from rat and 44 from human. In addition, FKPP synthesizes 25 potentially full-length mammalian kinesins based on the partial sequences in the database. Surprisingly, FKPP reveals that full-length human CENP-E contains 2701 aa rather than 2663 aa in the database. Experimentation using sequence specific antibody and cDNA sequencing of human CENP-E validates the accuracy of FKPP. Given the remarkable computing efficiency and accuracy of FKPP, we reclassify the mammalian kinesin superfamily. Since current databases contain many incomplete sequences, FKPP may provide a novel approach for molecular delineation of kinesins and other protein families.

关 键 词：驱动蛋白 基因组 CENP-E FKPP 

分 类 号：Q78[生物学—分子生物学]