低氧对大鼠附睾功能的影响  

Effect of hypoxia on epididymis of rat

作　　者：廖卫公[1] 高钰琪[1] 吴艺[1] 蔡明春[1] 范有明[1] 

机构地区：[1]第三军医大学高原军事医学系病理生理学与高原生理学教研室,全军高原生理与高原疾病研究重点实验室,重庆400038

出　　处：《生殖医学杂志》2006年第4期252-256,共5页Journal of Reproductive Medicine

摘　　要：目的研究低氧对大鼠附睾功能的影响。方法将成年雄性Wistar大鼠随机分为常氧和低氧5d、15d、30d4组。低氧各组置低压舱内模拟5,000m高原低氧。测定附睾尾精子数量、质量,生化法测定各组大鼠附睾液中果糖、肉毒碱的含量及α-葡萄糖苷酶的活性。结果低氧15d组、30d组大鼠附睾尾精子数显著低于常氧组。低氧各组精子活力、活率显著低于常氧组;尾部畸形精子数显著高于常氧组。低氧5d组、15d组大鼠附睾液果糖含量显著高于常氧组(P<0.01)。低氧5d、30d组,大鼠附睾液中性α-葡萄糖苷酶活性显著低于常氧组。结论低氧可抑制附睾尾精子数量和质量,并抑制附睾液中性α-葡萄糖苷酶活性,提示低氧可干扰附睾功能,并抑制精子的成熟。Objective： To explore the effects of hypoxia on the epididymis of rat. Methods： Adult male Wistar rats were randomly divided into four groups： control group raised at sea level; 5 d, 15 d and 30 d hypoxic group raised in a hypobaric chamber simulating 5,000 m altitude for 5 days, 15 days and 30 days, respectively. Sperm number, viability, motility and morphologically abnormal sperm were measured in epididymal caudal. Fructose, L-carnitine level and neutral α-glucosidase activity were also determined in epididymal fluid. Results： Sperm concentrations in hypoxic 15 d, 30 d group were lower than that in control group （P〈0.05）. Sperm motility and sperm viability in three hypoxic groups were lower in comparison to those in control group （P〈0.01）. Hypoxia induced much abnormal sperm. In hypoxic 5 d group and hypoxic 30 d group, neutral α-glucosidase activities in epididymal fluid were significantly lower than those in control group （P〈0.05）. Conclusion. Hypoxia disturbs the function of epididymis and inhibits rat sperm maturation.

关 键 词：低氧 附睾 果糖 肉毒碱 Α-葡萄糖苷酶 

分 类 号：R339.2[医药卫生—人体生理学]