PDGF-BB对成年雌性大鼠成骨细胞雌激素受体α蛋白质表达影响的实验研究  

Effect of PDGF-BB on expression of estrogen receptor α protein of osteoblast in mature female rats

作　　者：荣杰生[1] 陶树清[1] 张滨[1] 陶天遵[1] 刘伟[2] 张淑云[2] 杜博[2] 金茜[2] 

机构地区：[1]哈尔滨医科大学附属第二医院骨外二科,150086 [2]哈尔滨医科大学附属第二医院科研实验中心,150086

出　　处：《中国骨质疏松杂志》2006年第4期338-341,345,共5页Chinese Journal of Osteoporosis

摘　　要：目的通过研究PDGFBB与ERα的关系,探讨PDGFBB在骨代谢中的作用机理,并对其在骨质疏松症中的应用前景进行展望。方法①利用酶消化法分离培养成年雌性大鼠的成骨细胞;②对培养的成骨细胞及其雌激素受体α进行鉴定;③分别加入浓度为0ngml、0.1ngml、1ngml、10ngml的PDGFBB,共同培养7d,并设阳性对照组和阴性对照组;④Westernblotting化学发光法检测ERα蛋白质,GIS2010凝胶图象处理系统分析结果。结果①培养的细胞为成骨细胞,免疫组化显示ERα位于胞核;②SDSPAGE电泳显示在66kD处有清晰的蛋白条带;③Westernblot显示10ngml组、1ngml组、0.1ngml组ERα蛋白条带较0ngml组清晰,且10ngml组最明显。结论PDGFBB具有促进成骨细胞雌激素受体α蛋白表达的作用,雌激素受体α蛋白含量随着PDGFBB浓度的增加而呈增加的趋势。Objeetive To investigate the relationship between PDGF-BB and estrogen receptor-α （ER-α） and to elucidate the mechanism of the role of PDGF-BB in bone metabolism and the prospect of PDGF-BB in treatment of osteoporosis （OP）. Methods The osteoblasts of adult female rat were separated by enzyme-digesting method and cultivated and its ER-a were identified. Different concentrations of PDGF-BB （0 ng/ml, 0. 1 ng/ml, 1 ng/ml, 10 ng/ml） were applied to the cultural media for 7 days. The positive and negative contrast group were set for ER cells. ER-α protein was examined by Western blot. Results （ 1 ） The cells cultured were identified as osteoblast and ER-α was found in the nucleous by immunohistochemistry. （2） In SDS-PAGE electrophoresis, a clear protein stripe at 66kD was shown. （3） The protein stripes in Western blot in the group of 0. 1 ng/ml, 1 ng/ml, 10 ng/ml were clearer than that of the group of 0 ng/ml and was the most in the group of 10 ng/ml. Conclusions PDGF-BB can increase the expression of ER-α in osteoblast and the concentration of ER-α protein increased with that of PDGF- BB.

关 键 词：成骨细胞 雌激素受体Α 血小板衍生生长因子BB 蛋白质表达 

分 类 号：R737.9[医药卫生—肿瘤] R543.502[医药卫生—临床医学]