食管、贲门癌和癌前病变组织中IMP1蛋白的表达变化  

Alterations of IMP1 protein in esophageal and gastric cardia cancer and precancerous lesions

作　　者：杜芳[1,2] 王立东[1] 焦新英 孙哲[1] 高珊珊[1] 范宗民[1] 何欣[1] 郭涛[1] 齐义军[1] 余炜伟[1] 王智卿[1] 江亚南[1] 常志伟[1] 李吉林 常扶保 郭花芹[1] 尹艳春[1] 牛牧[1] 李江曼[1] 

机构地区：[1]河南省食管癌重点开放实验室,郑州大学医学实验中心癌症研究室,郑州大学第一附属医院,郑州450052 [2]中国人民解放军第一五三医院消化内科,林州456592 [3]河南省林州市姚村食管癌医院病理科,林州4565504 [4]河南省林州市中心医院胸外科

出　　处：《临床与实验病理学杂志》2006年第4期425-428,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家杰出青年科学基金(30025016);河南省高校创新人才工程基金(1999125);河南省医学创新人才工程项目(200084);河南省食管癌重点开放实验室基金(20050227);郑州大学211工程项目

摘　　要：目的探讨胰岛素样生长因子ⅡmRNA结合蛋白IMP1在食管癌和贲门癌及癌前病变组织的表达变化特征及其生物学意义。方法采用免疫组化ABC法检测123例食管和贲门癌及癌前病变组织IMP1蛋白的表达状况和特征。结果食管:IMP1在正常鳞状上皮组织表达最高(100%),并随病变进展,阳性表达率呈下降趋势,正常食管上皮与基底细胞过度增生(BCH,91·67%)、不典型增生(DYS,85·71%)和鳞状细胞癌(SCC,83·33%)之间差有异显著性(P<0·05),且BCH与SCC之间阳性表达率差异明显(P<0·05)。贲门:IMP1在各级病变组织均有不同程度表达,不典型增生组织(DYS,83·33%%)阳性率最高,表达最低是慢性萎缩性贲门炎组织(CAG,54·55%),DYS与正常贲门腺上皮(61·11%)、CAG和贲门腺癌(GCA,61·90%)之间差异有显著性(P<0·01)。IMP1表达率与食管鳞癌和贲门腺癌分化程度无相关性(P>0·05)。结论IMP1蛋白表达变化是食管癌、贲门癌和癌前病变组织常见的分子事件,可能在食管鳞癌和贲门腺癌癌变过程中起一定作用。Purpose To evaluate the significance of IMP1 protein expression in esophageal cancer, gastric cardia cancer and precancerous lesions. Methods Immunohistochemical staining （ ABC method） was undertaken to determine the expression of IMP1 proteins in 123 cases of esophageal cancer and gastric cardia cancer and precancerous lesion. Results Esophagus ： the positive rate of IMP1 protein was the highest in normal esophageal epithelia（ NOR, 100% ） , which positive rate declined in the progress of lesion, and there was significant difference（P〈0.05） between NOR and based cell hyperplasia （BCH,91.67%）, NOR and dysplasia （DYS, 85.71% ）, NOR and squamous cell carcinoma（ SCC, 83.33% ）, BCH and SCC, respectively. Gastric cardia： IMP1 expressed in all grades of lesions. 83.33% in dysplasia （DYS）, 54. 55% in chronic atrophy gastritis （CAG）, and there was significant difference be- tween DYS and normal epithelia（61.11% ） （P 〈0. 05）, CAG and GCA（61.90% ） （P 〈0.01 ）. And there was not significant relationship between the positive rate of IMP1 and differentiation extent of SCC and GCA （ P〉0.05 ）. Conclusions The present results demonstrate that the change of IMP1 protein expression is a frequent molecular event in esophageal and gastric cardia precancerous and cancerous lesions, which indicates that the alterations of IMP1 may play an important role in esophageal and gastric cardia carcinogenesis.

关 键 词：食管肿瘤 贲门肿瘤 鳞状细胞癌 腺癌 癌前病变 胰岛素样生长因子ⅡmRNA结合蛋白 

分 类 号：R735.1[医药卫生—肿瘤] R341.3[医药卫生—临床医学]