维生素C与维生素E的联合免疫调节及抗氧化作用  

Effects of vitamin C and vitamin E combination on immunoregulation and antioxidation

作　　者：朱长林[1] 张文彬[1] 孙鹏[1] 周启立[1] 敬小青[1] 刘霞[1] 侯文敏[1] 

机构地区：[1]承德医学院附属医院儿科,河北省承德市067000

出　　处：《中国临床康复》2006年第36期120-122,共3页Chinese Journal of Clinical Rehabilitation

基　　金：卫生部国际合作司主管的笹川医学奖学金同学会科研启动基金资助项目(097);2005年河北省科技攻关计划项目(052761953);2005年承德市科技攻关计划项目(200522020)~~

摘　　要：目的:观察维生素C和维生素E联合应用对小鼠免疫功能及抗氧化功能的调节作用。方法:实验主要于2004-04/2005-10在承德医学院附属医院中心实验室完成,部分实验在日本千叶大学完成。选择雌性清洁级BALB/c小鼠60只,按随机数字表法分成6组,每组10只。分别为:①正常对照组:每天灌胃生理盐水0.3mL。②联合维生素组:每天灌胃0.3mL生理盐水内含维生素E0.3mg、维生素C1.5mg。③免疫抑制组:小鼠每5d腹腔注射环磷酰胺0.6mg(30mg/kg),共4次,制备免疫功能低下模型。④免疫抑制+维生素C组:每天灌胃0.3mL生理盐水内含维生素C1.5mg。⑤免疫抑制+维生素E组:每天灌胃0.3mL生理盐水内含维生素E0.3mg。⑥免疫抑制+联合维生素组:每天灌胃0.3mL生理盐水内含维生素E0.3mg、维生素C1.5mg。后4组小鼠均制备免疫功能低下模型,方法同免疫抑制组。连续给药20d后,麻醉小鼠断尾取血,处死后取其脾脏、肝脏及肌肉。通过测定小鼠刀豆蛋白A刺激淋巴细胞转化功能(计算刺激指数,刺激指数与淋巴细胞转化能力成正比)、脾细胞产生白细胞介素2的反应活性、天然杀伤细胞活性(酶标仪490nm处测定吸光度值(A),杀伤活性=(A实验组-A总自然释放)/(A正常对照组-A总自然释放)×100%)、血清及肝组织中脂质过氧化物含量、肝脏超氧化物超氧化物活性等指标,评估小鼠免疫功能和抗氧化功能的变化情况。结果:实验过程中免疫抑制组有1只小鼠在给药第17天死亡。①各组小鼠淋巴细胞刺激指数比较:免疫抑制组显著低于正常对照组(59.2%,100%,P<0.01),免疫抑制+联合维生素组显著高于免疫抑制组(105.2%,P<0.01)。②各组小鼠天然杀伤细胞活性比较:免疫抑制+联合维生素组小鼠的天然杀伤细胞活性显著高于免疫抑制组(P<0.01)。③各组小鼠白细胞介素2生成率比较:免疫抑制+维生素E组及免疫抑制+联合维生素组均显著高于免疫抑制�AIM： To observe the regulatory effects of vitamin C and vitamin E combination on immune function and antioxidant in mice. METHODS： The experiment was conducted mainly at the Central Laboratory, Affiliated Hospital, Chengde Medical College from April 2004 to October 2005. Partial experiment was performed at Japanese Qianye University. Totally 60 female BALB/c mice of clean grade were selected and randomly assigned into 6 groups with 10 in each group.①The mice in the normal control group received 0.3 mL saline by gastric perfusion every day. ②The mice in the vitamin combination group were treated with 0.3 mL saline containing 0.3 mg vitamin E and 1.5 mg vitamin C by gastric perfusion every day. ③The mice in the immune suppression group were given 0.6 mg （30 mg/kg） cyclophosphamide by intraperitoneal injection every 5 days for 4 times so as to establish low immune function models. ④ The mice in the immune suppression plus vitamin C group were given 0.3 mL saline containing 1.5 mg vitamin C by gastric perfusion every day.⑤ The mice in the immune suppression plus vitamin E group were given 0.3 mL saline containing 0.3 mg vitamin E by gastric perfusion every day. ⑥The mice in the immune suppression plus vitamin combination group were treated with 0.3 mL saline containing 0.3 mg vitamin E and 1.5 mg vitamin C by gastric perfusion every day. The mice in the latter 4 groups were made into low immune function model, and the method was the same to the immune suppression group. After successive administration for 20 days, blood was taken out from the tail of anesthetized mice. After the mice were killed, spleen, liver and muscles were isolated. Changes of immune function and antioxidation in mice were assessed by measuring lymphocyte transformation function （calculation of stimulation indices, stimulation indices was in direct proportion to lymphocyte transformation ability） stimulated by canavaline A of mice, reaction activity of splenic cell producing interleukin 2, activity of natural killer cells

关 键 词：抗坏血酸 维生素E 免疫活性 抗氧化剂 

分 类 号：R446.6[医药卫生—诊断学]