低浓度和高浓度1,6-二磷酸果糖对白细胞介素1β损伤胰岛细胞的影响(英文)  

Effects of fructose-1,6-diphosphate of low and high dosages on islet cells damaged by interleukin-1 beta

作　　者：郑倩[1] 黄丹丹[2] 曹弟勇[3] 敬华娥[2] 蓝海涛[2] 刘华[2] 刘红[2] 

机构地区：[1]川北医学院生理教研室,四川省南充市637007 [2]四川大学华西基础医学与法医学院,四川省成都市610041 [3]川北医学院药理教研室,四川省南充市637007

出　　处：《中国临床康复》2006年第36期185-187,共3页Chinese Journal of Clinical Rehabilitation

基　　金：四川省教育厅重点项目资助(2005A082)~~

摘　　要：背景:一定浓度1,6-二磷酸果糖对白细胞介素1β损伤的胰岛具有保护作用,低浓度和高浓度的1,6-二磷酸果糖对白细胞介素1β损伤的胰岛具有不同的作用。目的:探讨低浓度和高浓度1,6-二磷酸果糖对白细胞介素1β损伤胰岛细胞的影响。设计:分组设计、对照动物实验。单位:川北医学院生理教研室。材料:实验于2004-07/2006-02在川北医学院外科肿瘤实验室和风湿免疫中心完成。选择出生1～3d的Wistar大鼠20只。方法:取鼠的胰腺,收集胰岛细胞,分为正常对照组、白细胞介素1β损伤组、白细胞介素1β+1,25,50mmol/L1,6-二磷酸果糖组。应用四唑盐比色法检测细胞活性;放射免疫法检测胰岛素的基础和高糖分泌量;用一氧化氮和一氧化氮合酶试剂盒分别检测各组一氧化氮含量和一氧化氮合酶活性;采用Fura-2荧光检测技术测定各组[Ca2+]i。主要观察指标:检测胰岛细胞活性,胰岛素的基础和高糖分泌量,一氧化氮含量和一氧化氮合酶活性,[Ca2+]i浓度。结果:①白细胞介素1β损伤组、白细胞介素1β+1,25,50mmol/L1,6-二磷酸果糖组胰岛细胞活性(A值)明显低于正常对照组(分别为0.116±0.012,0.129±0.008,0.125±0.015,0.120±0.016,0.252±0.020,P<0.01);1,6-二磷酸果糖浓度过低(1mmol/L)或过高(25,50mmol/L)时胰岛细胞活性(A值)与白细胞介素1β损伤组比较差异无显著性意义(P>0.05)。②白细胞介素1β损伤组、白细胞介素1β+1,25,50mmol/L1,6-二磷酸果糖组胰岛细胞基础分泌胰岛素量和葡萄糖刺激分泌量显著低于正常对照组[分别为(237.00±22.21),(230.83±11.58),(225.16±12.46),(220.50±15.63),(425.67±16.85)mIU/L;(90.17±6.11),(96.62±8.64),(87.66±8.24),(85.46±9.59),(204.50±10.78)mIU/L,P<0.01],而低、高浓度1,6-二磷酸果糖组与白细胞介素1β损伤组比较差异无显著性意义(P>0.05)。③白细胞介素1β损伤组细胞上清液中一氧化氮合酶活性与一氧化氮含量明显高于正�BACKGROUND： Fructose-1,6-diphosphate （FDP） of certain dosage plays a protective role in the pancreatic islets damaged by interleukin-1 beta （IL 1β）, and there are different effects of FDP of low and high dosages. OBJECTIVE： To investigate the effects of FDP of low and high dosages on the islet cells damaged by IL-1β. DESIGN： A grouped design and controlled animal experiment. SETTING： Department of Physiology, North Sichuan Medical College, MATERIALS： The experiments were carried out in the Tumor Laborator and Central Laboratory of Rheumatology and Immunology, Department of Surgery, North Sichuan Medical College between July 2004 and Februar 2006. Twenty Wistar rats within 1-3 days after birth were selected. METHODS： The pancreases of the rats were removed to collect islet cells, and then the cells were divided into normal control group, IL-1β damaged group, IL-1β＋1, 25, 50 mmol/L FDP groups. The cellular activity was detected with methyl-thiazol-tetrazolium （MTT） assay, basic amount of insulin secretion and that stimulated by high glucose with radioimmunoassay, content of nitric oxide and activity of nitric oxide synthase （NOS） with nitric oxide and NOS kits, and the with [Ca^2＋]i with Fura-2 fluorescent assay. MAIN OUTCOME MEASURES： Activity of islet cells; basic amount of insulin secretion and that stimulated by high glucose; content of nitric oxide and activity of NOS; [Ca^2＋]i. RESULTS： ① The activities （A values） of the islet cells in the IL-1β damaged group, IL-1β＋1, 25, 50 mmol/L FDP groups were obviously lower than that in the normal control group （0.116±0.012, 0.129±0.008, 0.125 ±0.015, 0.120±0.016, 0.252±0.020, P 〈 0.01）. The activities （A values） of the islet cellswere not significantly different from that in the 1L-1β damaged group （P 〉 0.05） when the FDP dosage was too low （1 mmol/L） or too high （25 mmol/L）. ② The basic amount of insulin secretion and that stimulated by glucose were significantly lower in the IL-1β d

关 键 词：胰岛 细胞保护 果糖二磷酸盐类 白细胞介素1 

分 类 号：R322.51[医药卫生—人体解剖和组织胚胎学]