HBV基因型及A1762T/G1764A双位点变异在肝癌患者中的临床意义  

Clinical study on the relationships between HBV genotype and HBV basic core promoter mutants in HCC patients

作　　者：丁红芳[1] 田德英[1] 杨道锋[1] 皮斌[1] 许东[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院感染科,湖北武汉430030

出　　处：《中西医结合肝病杂志》2006年第4期206-209,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:研究肝细胞癌(HCC)患者乙型肝炎病毒(HBV)基因型与基本核心启动子(BCP)基因区A1762T/G1764A双位点变异(BCP区双突变)之间的关系,探讨HCC的发病机制。方法:选择40例HCC患者作为研究组,40例慢性乙型肝炎(CHB)患者作为对照组,采用多对型特异性引物PCR扩增法进行基因分型及HBV基因多态性芯片检测BCP区A1762T/G1764A双位点变异。结果:40例HCC患者中有30例HBV DNA定量阳性,平均对数值为(6·53±1·31)copy/ml,将30例HBV DNA定量阳性的HCC及40例CHB患者的血清进行HBV基因分型及基因变异检测,结果显示30例HCC中HBV B基因型5例(16·7%),C基因型25例(83·3%);BCP区双突变共有20例(67·7%),其中B基因型1例,C基因型19例,BCP双突变率在B基因型和C基因型中分别为20%(1/5)和76%(19/25);40例CHB患者中B基因型32例(80%),C基因型8例(20%);BCP区双突变共有13例,其中B基因型8例,C基因型5例,BCP双突率在B基因型和C基因型中分别是25%(8/32)和62·5%(5/8)。结论:肝细胞癌的发生与BCP区A1762T/G1764A双位点变异有关,多发生在HBV C基因型的患者。Objective： To investigate the relationships between hepatitis B virus （HBV） genotype and HBV core promoter mutants, and in order to explore the pathogenesis of hepatocellular carcinoma. Methods： Serum samples from 40 patients with hepatoma and 40 patients with chronic hepatitis B （CHB） were collected and tested for HBV genotypes by the multiplex typespecific primers, Signal-base mutations of G1896A. A1762T and G1764A were analyzed by gene chips technique. Results： There were 30 cases in the 40 HCC patients whose HBV DNA quantification were positive, the average levels of the HBV DNA quantification were （6. 53 ± 1.31 ） copy/ml （Logarithm） . The HBV genotypes and HBV mutants were tested for the 30 HCC and 40 CHB patients, the results showed that in the 30 HCC cases, 5 （ 16. 7% ） were genotype B, 25 （83.3%） were genotype C; Twenty of the 30 HCC patients were found to have mutants in HBV BCP region at nucleotide A1762T/G1764A, among which 1 case were detected with genotype B and 19 cases with genotype C （ 20% VS 76% , P 〈 0. 05 ） . In the 40 CHB patients, the percentagens of genotype B and C were 80%, 20% respectively, 13 of 40 CHB patients were found to have mutants in BCP region, among which 8 cases were genotype B and 5 cases with genotype C. Conclusion： The genotype C is the predominant genotype in HBV related HCC, and there are a clear link between HBV genotype and HBV BCP mutants at nucleotide A1762T/ G1764A in hepatoma.

关 键 词：肝细胞癌 HBV 基因型 BCP区双突变 

分 类 号：R735.7[医药卫生—肿瘤]