安徽IBV地方株AH1-99株S2基因的克隆与序列分析  

Cloning and Analysis of S2 Gene of Infectious bronchitis virus of Anhui Strain AH1-99

作　　者：何长生[1] 刘胜旺[1] 魏建忠[2] 朱良强[3] 詹松鹤[3] 江定丰[3] 郑举[3] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001 [2]安徽农业大学动物科技学院,安徽合肥230036 [3]安徽省兽医工作站,安徽合肥230022

出　　处：《动物医学进展》2006年第8期98-101,共4页Progress In Veterinary Medicine

摘　　要：用RT-PCR技术扩增出IBV AH1-99株的S2基因cDNA,并将其克隆到pMD18-T载体中进行序列测定和分析。结果表明,该分离株S2基因全长共1 881个核苷酸,编码625个氨基酸;与GenBank中登录的IBVS2基因核苷酸的同源性为72.5%~88.9%,氨基酸的同源性为70.3%~88.0%;在895 nt^900 nt处插入了GCG ACT 6个碱基,在1 441 nt^1 446 nt处缺失了AT-TACT 6个碱基。S2 gene cDNA of IBV AH1-99 strain was amplified by RT-PCR. The S2 gene fragment was cloned into pMD18-T vector, and was sequenced and analyzed. The S2 gene was composed of 1881 nucleotides and coded 625 amino acid residues. Compared with other of S2 genes IBV strains in GenBank , the homology of S2 gene nucleotides of AH1-99 was between 72.5% to 88.9%, and the homology of amino acid was 70.3% to 88.9% There was an insertion of GCGACT at the site of nucleotide 895-900, and a deletion of ATTACT at the site of nucleotides 1 441-1 446.

关 键 词：传染性支气管炎病毒 AH1—99株 S2基因 克隆 序列分析 

分 类 号：S858.31[农业科学—临床兽医学] S852.659.5[农业科学—兽医学]