神经干细胞移植促进脑出血大鼠神经功能恢复的实验研究  

Neural Stem Cell Transplantation Promotes Functional Recovery in Rats With Experimental Intracerebral Hemorrhage

作　　者：高俊玮[1] 罗湘颖[1] 伍军[1] 柳浩然[1] 卢明[1] 方芳[1] 方加胜[1] 

机构地区：[1]中南大学湘雅医院神经外科,长沙410008

出　　处：《卒中与神经疾病》2006年第4期208-211,共4页Stroke and Nervous Diseases

摘　　要：目的探讨脑内定向移植神经干细胞(NSCs)治疗脑出血的可行性和机制。方法采用在成年大鼠中立体定向纹状体内注入新鲜自体血液制作实验性脑出血模型,术后1d采用立体定向将在体外扩增、纯化并经BrdU标记的神经干细胞移植入脑出血部位,通过神经功能缺损评分来观察移植后大鼠神经行为学改善情况,并通过双标GFAP、NeuN、BrdU免疫组化来检测移植入脑的NSCs。结果移植的NSCs迁移至血肿周围区,并分化为神经元(≈10%BrdU阳性细胞)和星形胶质细胞(≈75%);与对照组比较,NSCs移植组在移植后1周有更好的神经功能缺损评分,而且此疗效可维持长达8周。结论移植的NSCs可在脑出血的大鼠脑中存活、迁移,并促进功能恢复。立体定向移植神经干细胞可改善实验性脑出血大鼠的神经功能。Objective To explore the feasibility and the mechanism of the intrastriatal administration of neural stem cells （NSCs） for treating intracerebral hemorrhage （ICH）. Methods Experimental ICH was induced by intrastriatal administration of autoblood culled from heart in adult rats. After purified, proliferated, and marked with BrdU, the NSCs were intrastriatally administrated into rats 1 day after surgery. Neurological Deficits Scores （NDS） were evaluated before and after experimental ICH. Transplanted NSCs were detected by immunohistochemistry with double-labeling of BrdU and GFAP/NeuN. Results Transplanted NSCs migrated selectively to the perihematomal areas and differentiated into neurons （≈10% of BrdU ＋ cells） and astrocyted （≈75%）. The NSC-transplanted group showed better functional performance after 1 weeks compared with the control group （P〈0.05）, and the effect persisted for up to 8 weeks. Conclusions Intrastriatally transplanted NSCs can survive in the rat brain with ICH, migrate, and improve functional recovery. Transplantation of NSCs can be used to restore neurological deficits in experimental ICH.

关 键 词：神经干细胞 脑出血 细胞移植 

分 类 号：R743.34[医药卫生—神经病学与精神病学]