舒林酸抑制胃癌BGC-823细胞生长的实验观察  

Inhibitory effect of sulindac on the growth of gastric cancer cells in vitro

作　　者：于东红[1] 周蕾[1] 王萍[1] 王启之[2] 承泽农[1] 

机构地区：[1]蚌埠医学院病理学教研室,安徽蚌埠233030 [2]蚌埠医学院附属医院消化科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2006年第5期450-453,F0004,共5页Journal of Bengbu Medical College

基　　金：安徽省教育厅自然科学研究资助(2003Kj257);蚌埠市2003年第一批科技项目(10)

摘　　要：目的:观察舒林酸对胃癌BGC-823细胞生长的抑制作用,探讨其作用机制。方法:将舒林酸设置不同的作用浓度和作用时间于人胃癌BGC-823细胞共培养。采用MTT比色法检测胃癌细胞抑制率,流式细胞术检测胃癌细胞周期分布,TUNEL法检测细胞凋亡率,免疫组化检测细胞增殖(k i-67)、凋亡抑制基因(survivin)及还氧合酶(COX-2)蛋白的表达。结果:舒林酸使胃癌BGC-823细胞的生长受抑制,出现G0/G1期比例增高,S期比例降低,同时使细胞凋亡率显著上升;而k i-67、survivin及COX-2蛋白表达阳性率显著降低,上述作用均呈时间和剂量依赖性(P<0.01)。结论:舒林酸可抑制胃癌BGC-823细胞生长,其机制涉及影响细胞周期分布、诱导细胞凋亡及抑制COX-2、k i-67及survivin蛋白的表达。Objective：To observe the inhibitory effect of sulindac on the growth of gastric cancer cells BGC-823 and its antineoplastic mechanisms. Methods：Human gastric cancer cells BGC-823 were cultured with various concentrations of sulindac for different time. The proliferation of BGC-823 cells was measured by MTT colorimetrie assay; and the cell cycle distribution was examined by flow cytometry. Cell apoptosis was determind by TUNEL. The expressions of ki457, survivin and COX-2 in cells were detected by immunohistochemical staining. Results： Sulindac inhibited proliferation of BGC-823 cells,increased the proportion of cells in the G0/G1 phase,decreased the proportion of cells in the S phase,induced apoptosis of the BGC-823 cell line and decreased the expressions of ki- 67,survivin and COX-2 in cells. All the effects were in a time-dependent and dose-dependent manner （ P 〈 0. 05 ）. Conclusions： Sulindac inhibits the growth of gastric cancer cells BGC-823 and the antitumor mechanism may be related to changing cell cycle distribution,inducing cell apoptosis and inhibiting the expression of COX-2 ,survivin and ki457.

关 键 词：胃肿瘤 舒林酸 增殖 凋亡 还氧合酶-2 

分 类 号：R735.2[医药卫生—肿瘤]