高糖、高饱和脂肪酸及高不饱和脂肪酸饮食对老年大鼠胰岛素抵抗的影响  

Effects of high sucrose,high saturated fatty acid and high unsaturated fatty acid diets on insulin resistance in aging rats

作　　者：周宇[1] 宋光耀[1] 高宇[1] 冯志山[1] 边艳珠[1] 

机构地区：[1]河北省老年医学重点实验室河北省人民医院,河北石家庄050051

出　　处：《中国老年学杂志》2006年第2期222-224,共3页Chinese Journal of Gerontology

基　　金：河北省自然基金资助项目(基金编号303552)

摘　　要：目的探讨不同类型高脂肪酸及高糖饮食对老年大鼠胰岛素抵抗(IR)的影响。方法用高不饱和脂肪酸(HUFA),高饱和脂肪酸(HSFA),高糖(HS)饲料饲养大鼠24 w,观察体重(BW)、总胆固醇(TC)、甘油三酯(TG)、空腹血糖(FBG)、空腹胰岛素(FINS)、游离脂肪酸(FFA)的变化,并以正常血糖高胰岛素钳夹技术的葡萄糖输注率(G IR)对大鼠IR进行评价。结果12 w和24 w G IR在不同实验组间出现显著差异(均P<0.01),HSFA组G IR最低,24 w时HUFA组、HSFA组、HS组及对照组(NC组)G IR与0 w相比分别下降51.6%、52.1%、34.2%、26.4%。各实验组BW、TG、TC、FFA、FBG、FINS与NC组相比有不同程度升高,经多元回归分析,G IR与FFA、TG呈显著负相关(均P<0.01)。结论HUFA、HSFA和HS饮食长期摄入均可诱导大鼠IR;TG及FFA的升高与IR的形成有明显相关;随月龄增长大鼠的G IR有显著下降趋势,但饮食结构的影响更重要。Objective To study the effect of high different type of fatty acid and high sucrose diet on insulin resistance （IR） in aging rats. Methods The rats fed by high saturated fatty acid, high unsaturated fatty acid and high sucrose for 24 weeks to observe the change of body weight （ BW）, total cholesterol （ TC）, triglyceride （ TG）, fasting blood glucose （ FBG）, fasting blood insulin （ FINS）, free fatty acid （FFA） and evaluate IR of aging rats by glucose infusion rate （GIR） of hyperinsulinemic euglycemic clamp technique. Results 1. There were significant difference in GIR among experimental groups at 12 and 24 weeks （ P 〈 0.01 ） with the lowest GIR in high saturated fatty acid （HSFA） group. 2. The GIR decreased by 51.6%, 52.1%, 34.2%, 26.4% at 24 weeks in high unsaturated fatty acid （HUFA） group, HSFA group, high sucrose （HS） group mad normal control （NC） group respectively than that at 0 week. 3. BW, TC, TG, FBG, FINS, and FFA were increased obviously in experimental groups than those in NC group. Multiple step,rise regressions showed that TG and FFA were negatively related to GIR （P 〈 0.01 ）. Conclusions 1. Long-term intake of HUFA, HSFA and HS diet could induce IR of aging rats. 2. Increased TG and FFA could be related to the development of IR. 3. GIR of aging rats might have significant descending trend with aging, especially diet composition.

关 键 词：饱和脂肪酸 不饱和脂肪酸 游离脂肪酸 胰岛素抵抗 葡萄糖输注率 

分 类 号：R587.1[医药卫生—内分泌]