大肠癌组织中微血管密度、Survivin和P53测定  

Detection of Survivin, P53 and MVD in human colorectal carcinoma tissue

作　　者：郭长青[1] 刘文静[1] 刘国永[1] 

机构地区：[1]郑州大学第一附属医院消化内科,郑州450052

出　　处：《郑州大学学报（医学版）》2006年第5期933-935,共3页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:探讨大肠癌组织中Survivin和P53基因的表达及其与血管生成的关系。方法:采用免疫组化SP法,检测51例大肠癌(直肠癌22例,结肠癌29例;Dukes分期:A期13例,B期14例,C期15例,D期9例;高分化16例,中分化25例,低分化10例)组织中Survivin、P53的表达及微血管密度(MVD)。结果:Survivin、P53的表达与大肠癌的部位无关(P>0.05),而与组织分化程度、Dukes分期相关(P<0.05)。Survivin、P53阳性的大肠癌组织MVD(36.56±7.81,35.44±10.42)明显高于阴性者(19.81±9.27,22.17±9.54)(P<0.05)。Survivin、P53均为阳性时MVD值最高(39.78±7.88),高于其他各组(P<0.05)。Survivin与P53的表达呈正相关(r=0.957,P<0.05),MVD与Survivin、P53同时阳性表达呈正相关(r=0.787,P<0.05)。结论:Survivin和P53在大肠癌的发生和发展中起重要作用,并与肿瘤血管生成有关。Aim ： To investigate the expression of Survivin and p53 gene and their relations with tumor angiogenesis in colorectal carcinoma tissue. Methods： Immunohistochemical SP method was used to detect the expression of Survivin and P53 and microvessel density （MVD） in 51 cases of colorectal carcinoma. Results： The positive expressions of Survivin and P53 were correlated with histologic differentiation and Dukes stage（P 〈 0.05 ）. The MVD in subgroups of survivin（ ＋ ） （36.56±7.81） and P53（ ＋ ）（35.44 ±10.42） were significantly higher than those in Survivin（ - ）（19.81 ±9.27）and P53（ -）（22.17 ±9.54）（P〈0.05）,respectively. MVD in the subgroup of both Survivin（ ＋）and P53（ ＋ ） （39.78 ± 7.88） was significantly higher than those in the other subgroups （ P 〈 0.05 ）. Survivin expression was positively correlated with P53 expression（ r = 0. 957 ,P 〈 0.05 ） , and MVD was correlated with both Survivin and P53 expression（ r = 0. 787, P 〈0.05 ）, Conclusion： Survivin and P53 gene play a key role in the development of colorectal carcinoma, and correlate with the tumor angiogenesis.

关 键 词：SURVIVIN P53 大肠肿瘤 肿瘤血管生成 

分 类 号：R735.3[医药卫生—肿瘤]