永生化的骨髓基质细胞移植对损伤脊髓的保护作用  

Protective Effects of Intracord Transplantation of Immortalized Bone Marrow Stem Cells on Spinal Cord Injury

作　　者：钟海虹[1] 布林[1] 陈继铭[1] 

机构地区：[1]广东医学院第一临床学院外科学教研室,广东湛江524001

出　　处：《实用临床医学（江西）》2006年第8期7-9,共3页Practical Clinical Medicine

摘　　要：目的:观察永生化的骨髓基质细胞(Bone marrow stem cells,BMSCs)移植对损伤脊髓的保护作用。方法:将脊髓横断伤SD大鼠模型,随机分为损伤对照组(A组)、BMSCs移植组(B组)、神经营养因子(Nerve growth fac-tor,NGF)、脑源性神经生长因子(Brain Derived nerve growth factor,BDNF)基因修饰后永生化的BMSCs移植组(C组)和正常对照组(D组)。术后24 h后每组8只动物取脊髓伤段标本,测水离子含量。其余动物第6、12周,每组8只动物进行爬坡试验,评价下肢运动功能及运动诱发电位(MEP)检测。结果:脊髓损伤(SCI)后组织水肿,Na+、Ca2+离子浓度升高,K+、Mg2+离子浓度降低。NGF、BDNF基因修饰后永生化的骨髓基质细胞脊髓内移植后显著改善这些变化,且使SCI后神经功能有显著恢复。结论:NGF、BDNF基因修饰后永生化的BMSCs脊髓内移植对SCI有保护作用。其机制可能与减少神经细胞离子失衡,改善细胞内环境有关。Objective：To investigate the protective effects of intracord transplantation of immortalized Bone marrow stem cells on spinal cord injury. Methods：Spinal cord injured rats were divided into 3 groups. Group C consisted of the rat with treated with the transplantation of modified NGF,BDNF genes BMSCs（after immortalized） ;group B of the rats with transplantation of BM- SCs without, immortalized; At 24 h after operation,8 rats of each group were killed and the injuered segment of the spinal cord was resected to be examined with atomic absorption spectrophotometry. At 6 weeks and 12 weeks after transplantation,the another rats were examined with neurological function test and MEP monitoring. Results：there was a significant increase of water contant and Na^＋ ,Ca^2＋ions and decreased of K^＋ ,Mg^2＋ ions in the injured cord segment of group C and a statistically significant recovery was observed in group C. Conclusion： The transplantation of immortalized BMSCs（modified of NGF,BDNF genes） exert protective effects on injured segment of spinal cord through the improvement of the inernal ion enviroment of spinal cord.

关 键 词：永生化的骨髓基质细胞 神经营养因子 脊髓损伤 移植 

分 类 号：R-332[医药卫生]