发酵液中纳他霉素的提取及鉴定  

Extraction and Identification of Natamycin from Fermented Broth

作　　者：康健[1] 王敏 杜连祥[1] 

机构地区：[1]天津科技大学生物工程学院天津市工业微生物重点实验室,天津300222

出　　处：《药物生物技术》2006年第4期293-298,共6页Pharmaceutical Biotechnology

基　　金：天津市自然科学基金资助项目(项目编号:013609511);Hyflux膜应用研究基金项目

摘　　要：通过对纳他霉素部分理化性质的研究,建立了溶剂萃取法从发酵液中提取纳他霉素的工艺路线和参数。发酵液经预处理后离心分离收集菌体,加入适量甲醇,调节pH至10.5,离心除去不溶杂质,再调节pH至中性,经蒸发浓缩结晶获得纳他霉素。纳他霉素粗品经进一步纯化后,通过紫外光谱、红外光谱、核磁共振图谱和生物活性检测法进行了分析鉴定。通过该工艺回收的纳他霉素粗品纯度为74.6%±2.95%,回收率达到80.3%±3.27%,较好的保持了其生物活性。结果显示,该工艺在低浓度发酵液中提取纳他霉素达到较为理想的效果。The optimal parameters for extracting natamycin were investigated by studying the physical and chemical properties of natamycin. The process comprises pretreating the fermentation broth, collecting biomass by centrifugation, adding an amount of methanol, dissolving the natamycin in the broth sufficiently, adjusting pH 10. 5, removing the insoluble solids, lowering pH to neutral and vacuum-condensing the broth to precipitate the natamycin crystal. After a further purification, natamycin was identified by ultraviolet spectroscopy, infrared spectra, nuclear magnetic resonance and bioassay. The crude natamycin had good bioactivity, and the purity and recovery rate would be 74. 6% 2.95% and 80. 3% 3. 27% respectively. The results showed that this technique was suitable for natamycin extraction in low concentrations.

关 键 词：纳他霉素 萃取 紫外光谱 红外光谱 核磁共振 

分 类 号：Q939.97[生物学—微生物学]