HPLC法测定九味合剂中阿魏酸的含量  

Determination of Ferulic acid in Jiuwei Mixture by HPLC

作　　者：吴杨[1] 凌艳[1] 谢崇义[1] 李国忠[1] 

机构地区：[1]安徽省铜陵市人民医院,安徽铜陵244000

出　　处：《药品评价》2006年第4期297-298,共2页Drug Evaluation

摘　　要：目的建立九味合剂中阿魏酸的含量测定方法。方法采用高效液相色谱法,以TechsphereC18为色谱柱,流动相为甲醇—1%醋酸溶液(35∶65),检测波长为320nm。结果阿魏酸在9.36￣74.88!g·ml-1范围内,浓度与峰面积线性关系良好(r=0.9994),平均回收率为100.44%(n=6),RSD=1.63%。结论本法操作简便、快速,结果准确,可用于该制剂的质量控制。Objective To establish an HPLC method for determination of ferulic acid in Jiuwei Mixture. Methods The HPLC was performed on Techsphere C18 column with the mobil phase containing methanol-1% acetic acid solution（35：65）and set the UV director at 320nm. Results There was a good linear correlation in the range of 9.36-74.88μg·ml^-1（r=0.9994）.The average recovery was 100.44%（n=6）,RSD=l.63%. Conclusions The method is simple,rapid,accurate and available for the quality control of Jiuwei Mixture.

关 键 词：九味合剂 阿魏酸 含量测定 

分 类 号：R286.0[医药卫生—中药学]