依达拉奉影响缺血性神经元TNF-α和TGF-β1表达过程的实验研究  

Effects of edaravone on the expression of TNF-α and TGF-β1 in neurons in the hippocampal CA1 of ICR mice following ischemia-reperfusion

作　　者：刘文旭[1] 石静萍[1] 

机构地区：[1]南京医科大学附属脑科医院神经内科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2006年第9期784-787,F0003,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京市医学科技发展项目资助(ZKX0201)

摘　　要：目的:观察依达拉奉(edaravone,E)对局部短暂性脑缺血再灌注模型ICR小鼠海马CA1区TNF-α和TGF-β1表达过程的影响并探讨其作用机制。方法:夹闭ICR小鼠左颈内动脉近端20min后恢复灌流,分别于第8,24,48h,4、7、14天取材并进行TNF-α和TGF-β1免疫组化染色和常规HE染色,比较光镜下假手术组、缺血/再灌注组(模型组)、治疗组(缺血/再灌注+依达拉奉组)海马CA1区TNF-α和TGF-β1表达细胞数和存活神经细胞数(平均灰度值表示)。结果:①治疗组和模型组海马CA神经细胞存在早期TNF-α表达,治疗组中表达较模型组低(8、24、48h,P<0.01)、4天后表达消失。②该区神经细胞的TGF-β表达过程各不相同:模型组早期表达低于假手术组(8、24、48h,P<0.01),晚期高于假手术组(4、7、14天,P<0.01);治疗组为持续高表达,早期显著高于模型组(8、24、48h,P<0.01),也显著高于假手术组(8、24、48h,P<0.01);晚期高表达与模型组相似,显著高于假手术组(7、14天,P<0.01)。③缺血后该区的神经细胞存活表现:模型组中存活细胞显著少于假手术组(7、1天,P<0.01),而治疗组神经细胞丢失不明显,存活细胞数高于模型组(7、14天,P<0.01)、与假手术组差别无显著性(7、14天P>0.05)。结论:早期依达拉奉治疗显著改变局灶性短暂脑缺血再灌注小鼠海马CA1区TNF-α和TGF-β1表达过程,降低TNFα的早期活性表达,提高TGF-β1早期表达水平,且神经细胞无明显丢失,有脑保护意义。依达拉奉治疗作用可能和缺血/缺氧后神经细胞TNF-α和TGF-β1活性表达过程有关。Objective： To observe the effects of edaravone on the expression of TNF-α and TGF-β1 in the hippocampus CA1 of cerebral ischemia-reperfusion （I/R） mice and to explore the mechanism of brain protection of edaravone. Methods：Expressions of TNF-α,TGF-β1 in hippocampus CA1 zone from mice whose left internal carotid artery was reperfused after 20-min ischemia were analyzed by using immunohistochemistry staining and HE staining 8 h,24 h,48 h,4 d,7 d, 14 d later,respectively. Results： The early expression of TNF-α in this zone was significantly lower in the groups treated with edaravone than that in I/R group （8 h,24 h, 48 h, P 〈 0.01 ）. The expression of TGF-β1 in the group treated with edaravone was significantly higher than that in the sham operation group（P 〈 0.01）, and also higher than those in I/R group in the early stage（8 h,24 h,48 h, P 〈 0.01 ）.The number of nerve cells in CA1 zone was higher in the treated group than that in I/R group （7 d, 14 d, P 〈 0.01 ）. Conclusion：Edaravone plays a role in the process of expressions of both TNF-α and TGF-β1 of neurons in this zona of I/R mice. Edaravone might have protective effects on neurons.

关 键 词：脑缺血再灌注 小鼠 依达拉奉 细胞因子 线粒体 

分 类 号：R742[医药卫生—神经病学与精神病学]