雌二醇促进甲状腺乳头状癌细胞生长  

β-estrodiol promotes proliferation of papillary thyroid carcinoma cells

作　　者：沈美萍[1] 侯大卫[1] 刘翠萍[1] 武正炎[1] 

机构地区：[1]南京医科大学第一附属医院普外科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2006年第9期853-856,860,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省教育厅高校自然科学研究计划项目资助(02KB320004)

摘　　要：目的:探讨雌激素对甲状腺癌细胞生长的影响及其作用机制。方法:采用原代细胞培养获得甲状腺乳头状癌细胞,用不同浓度的β-雌二醇与甲状腺乳头状癌细胞共培养,4-甲基偶氮四唑蓝(MTT)法观察甲状腺癌细胞增殖情况,流式细胞仪DNA定量法分析雌二醇对甲状腺癌细胞细胞周期的影响,半定量RT-PCR法测定雌激素对甲状腺癌细胞CyclinD1mRNA表达的影响。结果:β-雌二醇刺激甲状腺乳头状癌细胞的增殖,并具有时间和浓度依赖性(P<0.05);β-雌二醇刺激甲状腺癌细胞后G0-G1细胞的比例减少,S期细胞增加(P<0.05);CyclinD1mRNA的表达升高(P<0.05)。结论:雌二醇促进甲状腺癌细胞从G1期向S期的转化而促进甲状腺癌细胞生长。Objective ： To investigate the effects of β-estrodiol on proliferation of thyroid papillary carcinoma cells, and to investigate to investigate the mechanisms possibly involved. Methods： The primary cultured papillary thyroid carcinoma cells were incubated with different concentrations of β-estrodiol and harvested at different times. The cell viabilities were measured through dimethyhhiazol-diphenyhetrazolium bromide （MTY） assay, and the cell cycles of the stimulated cells were analyzed by flow cytometer. The expression of cyclinD1 mRNA was observed by semi-quantitative RT-PCR. Results： β-estrodiol obviously promoted the proliferation of thyroid carcinoma cells in a dose-dependent and time-dependent pattern （P 〈 0.05）, which was inhibited by estrogen antagonist tamoxifen. After the treatment of β-estrodiol,the proportion of cells in G0-G1 phase decreased and cells in S phase increased significantly （P 〈 0.05）. The cyclinDl mRNA expression increased under 10-s mol/L β-estrodiol stimulation. Conclusion： β-estrodiol can promote the growth of papillary thyroid carcinoma cells by facilitating the G1/S phase transition.

关 键 词：甲状腺乳头状癌细胞 Β-雌二醇 CYCLIND1 

分 类 号：R736.1[医药卫生—肿瘤]