产肠毒素大肠杆菌k88ac基因原核融合表达载体的构建  

Construction of Prokaryotic Fusion Expression Vector of ETEC K88ac Gene

作　　者：解秋月[1] 栗庆丰[1] 庞严[1] 余丽芸[1] 

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2006年第4期51-53,共3页journal of heilongjiang bayi agricultural university

摘　　要：利用PCR技术扩增出菌毛K88ac基因片段,并将其插入到原核表达载体pET32a(+)的多克隆位点上。测序结果表明:与Genbank中序列比对同源性达到99%以上,成功构建了原核表达载体。为进一步的研究工作,奠定了基础。The gene K88ac was cloned by PCR, and was inserted into the multiple cloning sites of plasmid pET32a（＋）.The result of sequencing showed its homology was up to more than 99% compared with the sequence in Genbank, we successfully constructed the recombinant plasmid in prokaryocytic cell, and which provided the base for the further research work.

关 键 词：PCR 基因克隆 产肠毒素大肠杆菌 

分 类 号：S852.612[农业科学—基础兽医学] Q78[农业科学—兽医学]